维生素C体外诱导多能干细胞向心肌细胞的分化

背景：诱导多能干细胞治疗缺血性心脏病被认为是极具前景的方法,但目前仍未找到一种理想的诱导方式。目的：观察维生素C作为诱导剂对多能干细胞体外分化为心肌细胞的影响。方法：复苏小鼠诱导多能干细胞,传代培养后,采用直接悬浮培养法使小鼠诱导多能干细胞形成拟胚体,用含10-3,10-4,10-5,10-6mol/L4种不同浓度维生素C的分化培养基对其进行诱导分化,以不添加任何诱导剂作为对照组,观察各组出现搏动拟胚体的数量,计算分化比率。结果与结论：维生素C诱导小鼠诱导多能干细胞分化为心肌细胞的最佳浓度为10-4mol/L,有（57.00±3.20）%的拟胚体出现搏动,显著高于不添加任何诱导剂的对照组（5.13%±0.55）%（P〈0.01）。诱导而来的心肌细胞表达心肌特异性蛋白cTnT以及α-actin。提示最佳的维生素C诱导分化条件能够促进小鼠诱导多能干细胞向心肌细胞分化,提高其分化效率。

Vitamin C promotes differentiation of mouse pluripotent stem cells into cardiomyocytes in vitro

BACKGROUND：Inducing pluripotent stem cells have been considered promising treatment for ischemic heart disease. However,an ideal inducing method has not been found yet. OBJECTIVE：To investigate the effect of vitamin C on the cardiomyocytes differentiation from mouse induced pluriptent stem cells（miPS） . METHODS：miPS were recovered,passaged and form embryoid bodies. The embryoid bodies were induced by differentiation medium containing various concentration of vitamin C（10-3,10-4,10-5,10-6 mol/L） . Control group was not treated by inducer. The number of beating embryoid bodies were calculated. RESULTS AND CONCLUSION：The best concentration of vitamin C to promote the cardiac differentiation of miPS was 10-4 mol/L. Beating area was found in 57.00%±3.20% of embryoid bodies,significantly greater than control group（5.13%±0.55%;P 0.01） . Special cardiac proteins α-actin and cTnT were detected. Results showed that vitamin C could redound differentiation of miPS into cardiomyocytes.