脐血内皮祖细胞尾静脉与局部注射治疗糖尿病下肢缺血

背景：内皮祖细胞治疗糖尿病下肢缺血临床及动物实验多采用局部肌肉注射。目的：比较脐血内皮祖细胞鼠尾静脉与局部注射治疗糖尿病下肢缺血效果的差异。方法：取Wistar雄性大鼠分成5组：①糖尿病射线照射后结扎双后肢股动脉,尾静脉注射内皮祖细胞（DLV）。②糖尿病结扎双后肢股动脉左后肢局部肌肉注射PBS（DLC）,右后肢局部肌肉注射内皮祖细胞（DLM）。③正常大鼠射线照射后结扎双后肢,尾静脉注射内皮祖细胞（NLV）。④糖尿病不结扎不注射内皮祖细胞（DC）。用绿色荧光示踪内皮祖细胞,苏木精-伊红染色检测肌纤维间毛细血管数,RT-PCR检测双后肢肌肉血管内皮生长因子mRNA水平。结果与结论：DLV组与DLM组比较,右后肢腓肠肌溃疡及缺血好转明显,二者无明显区别;有明显荧光,差别不明显,Ⅷ因子免疫组织化学染色肌纤维间毛细血管数多,相互间无明显差别;腓肠肌血管内皮生长因子表达差异无显著性意义（P〉0.05）。提示脐血内皮祖细胞治疗糖尿病大鼠下肢缺血尾静脉注射与局部肌肉注射效果相当。

Intravenous and local injection of umbilical cord blood progenitor cells for treatment of diabetic lower limb ischemia

BACKGROUND： Local intramuscular injection of endothelial progenitor cells （EPCs） is often used in the treatment of diabetic lower limb ischemia as well as animal experiments.OBJECTIVE： To compare the intravenous and local injection of umbilical cord blood progenitor cells effects on diabetic lower limb ischemia.METHODS： Wistar male rats were divided into five groups： ①Diabetic rats with tail intravenous EPC transplantation following ligation of bilateral hind femoral artery （diabetes＋ligation＋intravenous injection,DLV）;②Diabetic rats with local EPC transplantation into the right hind limb following ligation of bilateral hind femoral artery （diabetes＋ligation＋intramuscular injection,DLM）;③Normal rats with tail intravenous EPC transplantation following ligation of bilateral hind femoral artery （normal＋ligation＋intravenous injection,NLV）;④Diabetic rats with ligation of bilateral hind femoral artery （diabetes＋ligation＋control,DLC）;⑤Diabetic rats without transplantation and ligation （diabetes＋control,DC）.EPCs were traced with green fluorescence,the capillary number among muscle fibers was measured using hematoxylin-eosin staining,and RT-PCR detection was performed for vascular endothelial growth factor （VEGF） mRNA level.RESULTS AND CONCLUSION： Gastrocnemius ulcers and ischemia in the Right hind limb was obvious improved in the DLV group as compared with DLM group,but there was no significant difference.The expression of fluorescence in the DLV and DLM groups was similar.The capillary number was higher in the DLV group than the DLM group,but there was no significant difference.VEGF mRNA levels had no difference between the DLV and DLM groups （P 0.05）.The findings indicate that the therapeutic effects of umbilical cord blood progenitor cells via tail intravenous and local injection are similar.