血管内皮生长因子及其受体在大鼠附睾内精子上的表达

背景：血管内皮生长因子及其受体在男性生殖领域中占有重要地位,但其在生殖系统中表达的意义和调节机制仍不十分清楚。目的：观察血管内皮生长因子及其受体类fms酪氨酸激酶在青春期大鼠附睾内精子上的表达定位情况。方法：取10只青春期雄性SD大鼠双侧附睾,分离得到浓度为（30～40）×109L-1的精子,涂片,免疫荧光法检测精子上血管内皮生长因子及其受体类fms酪氨酸激酶的表达定位情况。结果与结论：免疫荧光结果显示,血管内皮生长因子及其受体类fms酪氨酸激酶阳性蛋白均定位于大鼠附睾精子头部的顶体、尾部的颈段、中段和主段,尾部末段和精子核未见阳性染色。提示,血管内皮生长因子及其受体类fms酪氨酸激酶可能参与了精子的成熟过程,与精子的运动能力、获能和顶体反应有关。

Expression of vascular endothelial growth factor and its receptor in the epididymal sperm of adolescent male rats

BACKGROUND：Vascular endothelial growth factor （VEGF） and its receptor fms-like tyrosine kinase-1 （Flt-1） have a very important position in the field of male reproduction. However, it is still unclear about their expression meaning and regulatory mechanism in the reproductive system. OBJECTIVE：To study the expression and location of VEGF and Flt-1 in the epididymal sperm of adolescent male rats. METHODS：The expression of VEGF and Flt-1 was detected in 10 adolescent SD rats. The concentration of the sperm was （30-40）×109/L. Immunofluorescent staining was used for VEGF and Flt-1 expression and location in the sperm. RESULTS AND CONCLUSION：Immunofluorescent staining showed that VEGF and Flt-1 were both localized in the acrosome of sperm head, as well as in the neck, middle and principal segment of sperm tail. Specific expression patterns of VEGF and Flt-1 in the sperm of rats display that they may participate in spermiotelcosis, relevant to movement, capacitation and acrosome reaction of the sperm.