血根碱对人类宫颈癌细胞生长和转移的抑制作用

目的利用宫颈癌细胞株探讨血根碱（SAN）的抗肿瘤作用。方法采用MTT、克隆形成及细胞划痕试验研究不同浓度SAN对体外培养的人类宫颈癌HeLa和Siha细胞的影响。结果经1.0μmol/L和5.0μmol/LSAN处理后,Siha细胞的存活率分别为对照组的72%和10%（P〈0.01）,HeLa细胞比Siha细胞更敏感。用1.0μmol/LSAN处理后,HeLa和Siha细胞的克隆形成数从33个降低到14个和16个（P〈0.05）。划痕试验显示培养48h后,HeLa细胞和Siha细胞的“细胞伤口”宽度对照组分别为（0.51±0.04）mm和（0.64±0.02）mm,而0.5μmol/LSAN处理组为（1.22±0.02）mm和（1.63±0.01）mm（分别P〈0.01和P〈0.05）。MTT、克隆形成及细胞划痕试验均呈剂量和时间依赖性。结论SAN可抑制宫颈癌细胞的生长和转移。该研究为SAN作为一种新药应用于宫颈癌的治疗提供了初步的实验依据和理论基础。

Effect of Sanguinarine on Proliferation and Migration in Human Cervical Cancer Cells

Objective To study the anti-cancer effects of sanguinarine using two human cervical cancer HeLa and Siha cell lines. Methods MTT and colony formation were used to observe cell growth. Cell scratch test was performed to evaluate cell migration and invasion. Results After treatment with 1.0 μmol/L and 5.0 μmol/L SAN,the viability of Siha cells was 72% and 10%（P〈0.01）,respectively. HeLa cells were more sensitive than Siha cells. After treatment with 1.0 μmol/L SAN,colony formation in HeLa and Siha cells decreased from 33 to 14 and 16 （P〈0.05）. Cell scratch test showed cut width was （0.51±0.04） mm and （0.64±0.02） mm in the control and （1.22±0.02）mm and （1.63±0.01） mm in the HeLa and Siha cells treated with 0.5 μmol/L SAN ﹙P〈0.01 and P〈0.05﹚. MTT,colony formation and cell scratch test showed time-or dose-dependent manner. Conclusion SAN could inhibit the proliferation,migration and invasion of HeLa and Siha cells,which provide a laboratory evidence for potential application of SAN in clinical therapy of cervical cancer.