共刺激分子在Graves病甲状腺组织中的表达

目的探讨共刺激分子在Graves病（GD）的甲状腺组织中的表达及其免疫病理意义。方法运用细胞培养、流式细胞术和RT-PCR技术,检测10例GD组织和10例非毒性甲状腺肿（NTG）组织中共刺激分子基因的表达水平,原代培养的甲状腺滤泡细胞（TFCs）共刺激分子的表达及细胞因子对其表面共刺激分子表达的调节作用。结果GD组织一系列共刺激分子基因的表达明显高于NTG组,其中共刺激分子CD40、CD40L、OX40L和ICOS基因表达尤为显著;炎症类细胞因子可上调原代培养的TFCs表达共刺激分子CD40和GL50。结论共刺激分子在GD组织异常表达,提示CD40-CD40L、ICOS-GI50及OX40-OX40L信号在GD的局部免疫病理过程中起重要作用。

Expression of Costimulatory Molecule in Thyroid Tissue of Graves' Disease

Objective To study the expression of costimulatory molecule in thyroid tissue of patients with Graves＇ disease（GD） and its relationship to the pathogenesis of GD. Methods The thyroid tissues of patients were collected in surgical operation, including 10 cases of GD and 10 cases of nontoxic goiter （NTG）. The total RNA of these samples were extracted by Trizol. The gene expression of costimulatory molecule was assayed by RT-PCR and 1% agarose gel electrophoresis. Thyrocytes were cultured in the absence or presence of proinflammatory cytokines. The expression of costimulatory molecule on thyroid follicular cells （TFCs） was further measured by flow cytometry. Results The positive rates of costimulatory molecule gene expression in GD were relatively higher than in NTG, especially the levels of gene expressions of CD40, CD40L, OX40L and ICOS. Proinflammatory cytokines significantly increase the expres- sion of costimulatory molecule CIM0 and GL50 on TFCs. Conclusion Costimulatory signal CD40- CD40L, ICOS-GL50 and OX40-OX40L may exert important roles in the initiation and progression of auto- immune response through T cell mediated B cell activation.