Modulation of cytokine production from an EpiOcular corneal cell culture model in response to Staphylococcus aureus superantigen |
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| Authors: | A Thakur PhD A Clegg MSc A Chauhan MSc MDP Willcox PhD |
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| Institution: | Cooperative Research Centre for Eye Research and Technology and Cornea and Contact Lens Research Unit, School of optometry, University of New South Wales, Sydney, New South Wales, Australia |
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| Abstract: | The present study investigated the hypothesis that Staphylococcus aureus enterotoxin B (SEE) produces epithelial cell death and releases inflammatory cytokines that produce stromal infiltration during contact lens induced peripheral ulceration. Epithelial cells were incubated with different doses of SEB for various time periods. Culture supernatants were assayed for cytokines IL- lo, IL-6 and chemotactic agents IL-8 and LTB,. SEE induced the production of IL- I p and IL-8. Epithelial cells exposed for longer periods (48 h) with low concentrations of SEB produced significantly higher levels (N0.02) of IL-Ip and IL-8 (P<0.05) compared t o a 24 h exposure. SEB did not induce the production o f IL-6 and |
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| Keywords: | CLPU contact lens cytokines EpiOcular corneal model Stophyfococcus aureus |
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