白念珠菌多位点序列分型研究

目的研究白念珠菌的分子特征,建立分子流行病学研究方法—多位点序列分型技术(MLST),并对临床分离白念珠菌进行分型研究。方法对33株白念珠菌提取DNA,选择7对管家基因进行PCR扩增并将目的基因产物测序,然后将测序结果与标准序列比对后上传至数据库,获得相应的由7对管家基因组成的等位基因谱,最后将等位基因谱再次提交网站确认序列分型(sequence types,STs)。同时与ABC(ATP-binding cassette)分型结果进行比较。结果 33株临床白念珠菌通过MLST产生了33个STs,通过ABC分类为3型。结论 MLST具有较高的分辨力,是一种方便、快速的分子生物学方法,可用于流行病学和菌群多态性的研究。

Multilocus Sequence Typing of Candida Albicans

Objective To characterize different Candida albicans isolated through multilocus sequence typing(MLST),a molecular epidemiologic method was established.Methods Extract the DNA of 33 C.albicans isolated.Seven house-keeping genes were selected as target genes,and PCR was applied.Sequencing,analysis and comparison of the genes were conducted.The MLST results were compared with results obtained by ABC(ATP-binding cassette)-typing.Results Thirty-three isolated C.albicans were typed and 33 STs(sequence types)were found by MLST.ABC-typing produce three types.Conclusion The MLST assay had higher discrimination power.As a convenient and fast molecular typing method,MLST could be used to study the diversity of C.albicans and be applied to epidemiological research.