肺癌细胞和蛙皮素抑制树突状细胞的产生和功能

目的：了解肺癌细胞株及蛙皮素对树突状细胞（DC）产生及功能的影响。方法：树突状细胞由健康人外周血单个核细胞CD14^ ，在完全细胞培养液中加入1000U／ml GM-CSF和1000U／ml IL-4,培养7天获得，肺癌细胞株CRL－5815，CRL－5826，Bombesin和Bombesin受体拮抗剂加入培养液中，Annxin V检测DC凋亡；流式细胞仪检测CD40，CD86，CD83，CD80，HLA－DR阳性表达。结果：培养7－10天后的DC前体细胞表达高水平的HLA－DR CD80 CD86 CD83 CD40。肺癌及蛙皮素可导致DC前体细胞凋亡，而蛙皮素受体拮抗剂可部分保护蛙皮素致DC凋亡的作用。加入肺癌细胞株或蛙皮素与DC共培养时明显抑制上述细胞表型的表达和DC刺激同种异体T细胞的增殖能力，当加入蛙皮素受体拮抗剂时，DC细胞表达HLA－DR CD80 CD86 CD83 CD40明显增加，接近DC正常对照组。结论：肺癌细胞株及蛙皮素可导致DC的凋亡，抑制树突状细胞的产生和功能。

Lung cancer cells and bombesin inhibit dendritic cell generation and function

Objective To understand the effect of non-small cell lung cancer regulated dendritic cell function and generation. Methods CD14+ peripheral blood mononuclear cells (PBMC) obtained from human healthy volunteers. Cultured DC were collected and separately incubated with human lung cancer lines (CRL-5815, CRL-5826), bombesin and antagonist of bombesin in 6-well plates. Apoptosis of dendritic cells was analysed using Annexin V binding assay. Expressions of CD86, CD83, CD80, CD40, HLA-DR of DC were analyzed by FACscan. Ability of DC to stimulate proliferation of T cells was assessed with allogeneic mixed lymphocyte reactions (MLR) assay. Results Inhibition of DC generation by lung cancer: Dendritic cells from PBMC cultured with GM-CSF 1000U /ml and IL-4 1000U /ml for 7days expressed high levels of CD86, CD83, CD80, CD40, HLA-DR. Dendritic cells co-incubation with lung cancer cells and bombesin inhibited definitely expression of CD86, CD83, CD80, CD40, HLA-DR and significantly decreased ability to induce T cell proliferation (MLR). Antagonist of bombesin blockaded the function of lung cancer-induced apoptosis and generation of DC. Conclusion Non-small cell lung cancer (CRL-5815, CRL-5826) and bombesin inhibited dendritic cell maturation and function and induced apoptosis in vitro.