AA和MDS患者骨髓CD+34细胞表面GM-CSFR的表达及意义

目的 探讨再生障碍性贫血（AA）和骨髓增生异常综合征（MDS）患者骨髓CD34^＋细胞表面粒细胞-巨噬细胞集落刺激因子受体（GM—CSFR）的表达情况。方法 用流式细胞术（FCM）和单克隆抗体技术检测14例AA、23例MDS和8例非血液病患者骨髓CD34^＋细胞表面的GM—CSFR表达率。结果 MDS组骨髓CD34^＋细胞表面GM-CSFR的表达率明显高于对照组及AA组（P〈O．05），而AA组与对照组间差异无统计学意义（P〉0．05）。结论 AA患者造血干细胞没有质的缺陷；造血干细胞异常可能是MDS的主要发病机制之一。

Expression and value of GM-CSF receptors on CD34 positive cells in bone marrow of patients with aplastic and myelodysplastic syndrome

[Objective] To detect expression rate of granulocytemacrophage colony-stimulating factor receptors(GM-CSFR) on CD_(34) positive cells in bone marrow of patients with aplastic anemia(AA) and myelodysplastic syndrome(MDS).[Methods] The expression rates of GM-CSFR on CD_(34) positive cells in 14 AA patients, 23 MDS patients and 8 normal cases were detected by flow cytometry(FCM) and monoclonal antibody technique.[Results] The expression rate of GM-CSFR on CD_(34) positive cells in MDS group was higher than that in control group and AA group(P<0.05).There was no significant difference between AA group and control group(P>0.05).[Conclusion] It suggests that the quality of hematopoietic stem cells is normal in AA and the damaged quality of hematopoietic stem cells is one of the principal pathogenesis in MDS.