土贝母组织培养的研究

目的:研究土贝母茎段、叶片外植体愈伤组织的诱导和培养方法及其与土贝母皂苷积累的关系。方法:在MS或B₅培养基上,试验不同种类、不同含量的植物生长物质及其组合对土贝母茎段和叶片愈伤组织诱导和增殖的影响。结果:茎段愈伤组织诱导的最佳培养基配方是MS附加2,4-D 2.0 mg.L^-1,NAA 0.5 mg.L^-1和BA 1.0mg.L^-1;叶片愈伤组织诱导的最佳培养基配方是MS附加2,4-D 0.5 mg.L^-1和NAA 2.0 mg.L^-1。愈伤组织在MS附加2,4-D 2.0 mg.L^-1,NAA 0.5 mg.L^-1和BA 1.0 mg.L^-1的培养基上积累土贝母皂苷的含量最高;在B5附加2,4-D2.0 mg.L^-1,NAA 0.5 mg.L^-1和BA 1.0 mg.L^-1的培养基上经多次继代培养后,可形成较为均一、稳定的愈伤组织系。结论:建立了土贝母茎段、叶片外植体愈伤组织的诱导和培养方法,为植物组织细胞培养生产土贝母皂苷提供实验依据。

Study on tissue culture of Bolbostemma paniculatum

Objective: To study the method of callus induction and culturing of Bolbostemma paniculatum and its relationship with accumulation of tubeimoside.Method: Stem and leaf were selected as explants.The effects of growth substances and their combinations at different concentrations on callus formation and propagation on MS and B₅ basic medium were studied.Result: The optimal compositions of medium that induced calli from stems and leaves were the MS medium supplemented with 2,4-D 2.0 mg·L^-1,NAA 0.5 mg·L^-1 and BA 1.0 mg·L^-1and the MS medium supplemented with 2,4-D 0.5 mg·L^-1and NAA 2.0 mg·L^-1,respectively.The calli cultured on the MS medium supplemented with 2,4-D 2.0 mg·L^-1,NAA 0.5 mg·L^-1 and BA 1.0 mg·L^-1 were optimal for the accumulation of tubeimoside.A uniform and stable callus line could be formed from the calli cultured repeatedly on B_(5) medium supplemented with 2,4-D 2.0 mg·L^-1,NAA 0.5 mg·L^-1 and BA 1.0 mg·L^-1. Conclusion: Using stem and leaf as explants,the method of the callus induction and culture of B.paniculatum was established and it could provide some references for the production of tubeimoside by tissue and cell culture.