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| 小鼠着床前胚胎miRNA表达的实验研究 | |
| 引用本文： | 杨艳红,白文涛,张亮,尹国武,王晓红,王珺,张平,姚元庆. 小鼠着床前胚胎miRNA表达的实验研究[J]. 生殖医学杂志, 2008, 17(2): 123-129 |
| 作者姓名： | 杨艳红 白文涛 张亮 尹国武 王晓红 王珺 张平 姚元庆 |
| 作者单位： | 1. 第四军医大学唐都医院妇产科,陕西西安,710038 2. 第四军医大学基础部微生物学教研室,陕西西安,710032 3. 生物芯片北京国家工程研究中心暨博奥生物有限公司,北京,102206 |
| 摘 要： | 目的研究微小RNA(miRNA)在小鼠着床前胚胎各发育阶段的表达谱,探讨miRNA在着床前胚胎发育中的作用和意义。方法建立小鼠超排卵、交配和胚胎收集系统,采用miRNA扩增、miRNA芯片和荧光实时定量逆转录聚合酶链反应(RT-PCR)的方法研究小鼠着床前胚胎miRNA表达。结果小鼠卵母细胞、2细胞胚胎、4~8细胞胚胎和囊胚分别表达55、53、62和72个miRNA;四个发育阶段共筛查到表达94个miRNA,32个在各发育阶段均表达,62个在不同发育阶段特异性表达。实时荧光定量PCR方法验证mmu-miR-721在小鼠着床前胚胎中表达。结论小鼠着床前胚胎表达大量的miRNA,其可能对胚胎发育和胚胎细胞的分化起重要调控作用。 |
| 关 键 词： | miRNA 小鼠胚胎 基因调控 |
| 文章编号： | 1004-3845(2008)02-0123-07 |
| 修稿时间： | 2007-08-14 |
Expression of miRNA in mouse preimplantation embryos |
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| YANG Yan-hong,BAI Wen-tao,ZHANG Liang,YIN Guo-wu,WANG Xiao-hong,WANG Jun,ZHANG Ping,YAO Yuan-qing. Expression of miRNA in mouse preimplantation embryos[J]. Journal of Reproductive Medicine, 2008, 17(2): 123-129 | |
| Authors: | YANG Yan-hong BAI Wen-tao ZHANG Liang YIN Guo-wu WANG Xiao-hong WANG Jun ZHANG Ping YAO Yuan-qing |
| Affiliation: | YANG Yan-hong1,BAI Wen-tao2,ZHANG Liang3,YIN Guo-wu1,WANG Xiao-hong1,WANG Jun1,ZHANG Ping1,YAO Yuan-qing11.Department of Obstetrics & Gynecology,Tangdu Hospital,the Fourth Military Medical University,Xi'an 710038 2.Department of Microbiology,Xi'an 710032 3.National Engineering Research Center for Beijing Biochip Technology,Capital Bio Corperation,Beijing 102206 |
| Abstract: | Objectives: micro RNAs(miRNAs) are the newly found 19-25 nucleotide single stranded noncoding RNAs,which play important roles in gene regulation network and play critical roles in many biological processes including the regulation of development,cell proliferation or differentiation,apoptosis and cancer formation.In the present study,the expression of miRNAs at different stages of mouse preimplantation embryos was investigated.Methods: Mouse embryos(0.5-4.5 days p.c) were collected after superovulation.A me... |
| Keywords: | miRNA Mouse embryo Gene regulation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |