互隔交链孢霉提取物对淋巴细胞核仁形成区及细胞增殖周期的影响

本实验应用人类核仁形成区(NORs)选择性银染方法和细胞光度术,观察了互隔交链孢霉两菌株提取物261-B_2-3和C_(12)b_3-2对人淋巴细胞NORs活性的影响以及后者对细胞增殖周期移行的影响。结果显示,应用不同浓度的261-B_2-3和C_(12)b_3-2提取物处理细胞后,细胞携带Ag-NORs的染色体数明显减少,与对照组比较差异极显著(P<0.01)。表明两株提取物能明显抑制淋巴细胞NORs的活性。细胞暴露于不同浓度的C_(12)b_3-2提取物后,G_1%明显增多,S%和G_2+M%显著减少,与对照相比差异极显著(P<0.01)。说明C_(12)b_3-2提取物可能作用于G_1后期或S早期,延缓和阻滞了细胞周期的移行。

The effect of extracts of Alternaria alternata on nucleolus organizer region and cell proliferating cycle of lymphocytes

The authors have determined the effects of 261-B_2-3 and C_(12)b_3-2, extracts two strains of Alternaria alternata, on the nucleolus organizer regions (NORs) activity and the progression of cell proliferating cycle of human peripheral lymphocytes. The methods used in the experiment were selective silver staining of human NORs and cytophotometry. The result showed that the number of chromosomes bearing Ag-NORs was meaningly decreased when the cells were treated with different concentrations of 261-B_2-3 and C_(12)-b_3-2 extracts. The difference was quite significant when the data were compared with those of the control (P<0.01). It indicated that extracts of both stracts were able to suppress NORs activity of lymphocytes obviously. After the cells had been exposed to C_(12)b_3-2 extracts of different concentration, G_1% increased distinctly and S%, G_2+M% reduced significantly. The difference of data between the control and the treated group was very significant (P<0.01). It suggests therefore that C_(12)b_3-2 possibly acts at late-G_1 phase or carly-S phase and results in delay and block of the progression of cell cycle.