肺癌患者呼出气体中特征性挥发性有机化合物的研究

目的 筛选肺癌患者呼出气体中特征性挥发性有机化合物(VOCs),并探讨其对肺癌诊断的价值.方法 收集2007年2月至2009年8月在浙江大学医学院附属邵逸夫医院呼吸内科住院治疗的55例肺癌患者、21例肺部良性病变患者及30名住院健康体检者的呼出气体,采用固相微萃取联合气相色谱技术检测受试者呼出气体中的VOCs,比较分析各组的VOCs,筛选肺癌患者的特征性VOCs.结果 肺癌组45例检出庚醛(82%),肺部良性病变组1例检出庚醛(5%),健康组1名检出庚醛(3%),肺部良性病变组庚醛检出比例与健康组比较,差异无统计学意义(χ~2=0.06,P>0.05);将上述两组合并为非肺癌组,肺癌组庚醛检出比例明显高于非肺癌组(χ~2=65.06,P<0.05);庚醛的出峰情况与患者的年龄、性别、吸烟程度、肺癌分期及组织类型均无关(χ~2值为1.06～3.30,均P>0.05).结论 庚醛可能为肺癌患者的特征性VOCs之一,检测呼出气体中的VOCs对诊断肺癌可能具有一定的临床价值.

Measurement of exhaled volatile organic compounds in lung cancer patients

Objective To study the characteristics of volatile organic compounds (VOCs) in exhaled breath of lung cancer patients, and therefore to explore its use in the diagnosis of the disease. Methods From February 2007 to September 2009, 55 patients with lung cancer, 21 patients with benign lung diseases and 30 healthy controls were enrolled in our study. The VOCs in exhaled breath were detected by the SPME (solid phase microextraction)-GC (gas chromatography) system. Results Heptanal was detected in 45 patients of the lung cancer group, 1 of the benign lung disease group, and 1 of the healthy control group. The positive rate was higher in the lung cancer group as compared to the benign lung disease group and the healthy group combined (P<0. 05). The detection of heptanal was not related to age, smoking, histological type and staging (P> 0. 05). Conclusion Heptanal maybe a useful marker in VOCs from patients with lung cancer.