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| RP-HPLC法测定西咪替丁片中西咪替丁的含量 | |
| 引用本文： | 孙晓东. RP-HPLC法测定西咪替丁片中西咪替丁的含量[J]. 中国药房, 2011, 0(20): 1905-1906 |
| 作者姓名： | 孙晓东 |
| 作者单位： | 河北廊坊市人民医院; |
| 摘 要： | 目的:改进西咪替丁片中西咪替丁含量的测定方法。方法:采用反相高效液相色谱法。色谱柱为shimadzuVP-ODSC18柱,流动相为磷酸氢二钠溶液-甲醇(60∶40),流速为1.0mL·min-1,检测波长为215nm,进样量为10μL,柱温为30℃。结果:西咪替丁进样量在0.38～1.31μg(r=0.9999)范围内与峰面积积分值呈良好的线性关系;平均加样回收率为99.9%,RSD=0.54%(n=6)。结论:该方法简单,结果准确、可靠,可用于西咪替丁片的质量控制。 |
| 关 键 词： | 反相高效液相色谱法 西咪替丁片 含量测定 |
Content Determination of Cimetidine in Cimetidine Tablets by RP-HPLC |
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| SUN Xiao-dong. Content Determination of Cimetidine in Cimetidine Tablets by RP-HPLC[J]. China Pharmacy, 2011, 0(20): 1905-1906 | |
| Authors: | SUN Xiao-dong |
| Affiliation: | SUN Xiao-dong(Langfang People’s Hospital of Hebei Province, Langfang 065000, China) |
| Abstract: | OBJECTIVE： To improve the method for the content determination of cimetidine in Cimetidine tablets. METHODS： RP-HPLC method was used. The determination was performed on ODS-C18 column with mobile phase consisted of disodium hydrogen phosphate solution-methanol （60 ∶ 40） at flow rate of 1.0 mL·min-1. The detection wavelength was set at 215 nm and column temperature was 30 ℃. The injection volume was 10 μL. RESULTS： The linear range of cimetidine was 0.38～1.31 μg（r=0.999 9）with an average recovery of 99.9% （RSD=0.54%, n=6）. CONCLUSION： The method is simple, accurate and reliable. |
| Keywords: | RP-HPLC Cimetidine tablets Content determination |
| 本文献已被 CNKI 维普 等数据库收录！ | |