衰老对大鼠阴茎组织NO-cGMP通路及端粒酶活性的影响研究

目的探讨衰老对大鼠阴茎组织结构、NO(nitric oxide)-cGMP(cvclic Guanosine Monophosphate)通路及端粒酶活性的影响作用。方法本课题以不同月龄大鼠阴茎组织及培养的平滑肌细胞为研究对象,检测不同月龄大鼠阴茎组织中NO量、NOS(Nitric Oxide Synthase)活性、cGMP量、端粒酶活性及海绵体结构的变化,并比较大鼠、人阴茎组织及大鼠原代海绵体平滑肌细胞的端粒酶活性。结果(1)大鼠阴茎组织中NO量、NOS活性均先升高后降低,各月龄组间有显著差异。阴茎组织cGMP含量逐渐降低,各月龄组间差别显著；(2)随龄增加,平滑肌纤维逐渐减少,胶原纤维增多,粗大成团,窦状隙变少、变窄：(3)大鼠阴茎组织端粒酶活性以2月龄活性最高,随龄增加逐渐下降。人阴茎组织中无端粒酶活性。结论(1)衰老对大鼠阴茎组织结构、NO．cGMP通路及端粒酶活性有显著影响,提示衰老与ED关系密切；(2)大鼠阴茎组织有端粒酶活性,可作为研究细胞衰老与ED关系有关端粒酶的模型。

Study of the effects of aging on NO-cGMP pathway and telomerase activity in rat penile tissue

Objective To explore the effects of aging on NO-cGMP way,telomerase and component of penile tissue.Methods We had made some study on different month age(2,8,16,24month)rats and cultured smooth muscle cells,as follows:The NO,NOS activity,cGMP contents,the structure of penile tissue and telomerase activity were detected,the telomerase activity of SMC and penile tissue between rat and human were compared.Results(1)The NO content and NOS activity of penile tissue firstly rose(month 8,the highest)then dropped(month 24,the lowest), the cGMP contents decreased markedly;(2)The Masson staining showed that:smooth fibers decreased while collagen fibers increased;(3)The cultured SMC of rat had telomerase activity,and the level of telomerase activity of the month 2 was the highest in penile tissue.No telomerase activity in human penile tissue.Conclusion The effects of aging on NO-cGMP way,telomerase and component of penile tissue are significantly,which indicated that aging is correlated with ED tightly.Telomerase activity existed in rat penile tissue and not in human penile tissue,which indicated that rat can be used as the animal model about cell senescence and ED.