血小板衍化内皮细胞生长因子/胸腺嘧啶磷酸酶和血管内皮生长因子在间质性膀胱炎诊断中的应用

目的 检测血小板衍化内皮细胞生长因子/胸腺嘧啶磷酸酶(PDECGF/TP)和血管内皮生长因子(VEGF)的表达,探讨血管生长因子在间质性膀胱炎(IC)患者膀胱中的表达活性与膀胱镜下的表现及临床症状的关系.方法 选择符合NIH/NIDDK诊断标准的IC患者12例为实验组和12例Ⅲ型前列腺炎患者为对照组.实验组行麻醉下水扩张,膀胱组织活检,常规苏木素-伊红(HE)染色观察肥大细胞数量.应用免疫组织化学检测PDECGF/TP和VEGF表达,进行免疫组织化学阳性细胞计数及免疫组织化学评分(IHS).结果 实验组12名患者在麻醉下水扩张时发现膀胱黏膜点片状出血.常规HE染色病理切片观察可见IC组膀胱组织标本中肥大细胞数目明显高于对照组(P＜0.05),且IC患者的肥大细胞多存在于膀胱组织的黏膜下层,而对照组膀胱组织仅偶见肥大细胞.实验组患者膀胱黏膜PDECGF/TP和VEGF阳性细胞百分比计数、阳性细胞染色强度及HIS评分明显高于对照组(P＜0.05).结论 IC患者血管生长因子PDECGF/TP和VEGF呈高水平表达,通过检测PDECGF/TP和VEGF能够筛选或确诊间质性膀胱炎.

Abstract：

Objective To find out the relationship among the expression of vascular growth factors in patients with interstitial cystitis (IC) and their performance under the cystoscopy as well as the clinical symptoms by detecting the expression of platelet-derived endothelial growth factor/thymine phosphatase (PDECGF/TP) and vascular endothelial growth factor (VEGF). Methods Twelve cases of IC served as the experimental group and 12 cases of type Ⅲ as the control group. The patients in experimental group had bladder mucosa biopsy. The number of mast cells was observed by HE staining. The expression of PDECGF/TP and VEGF and immunohistological score (IHS) were detected by using immunohistochemistry. Results Bladder mucosa hemorrhage points and sheets were observed under water expansion. The number of mast cells existing in the submucosa of the bladder tissue was significantly greater than in control group (P ＜ 0. 05 ). The percentage of PDECGF/TP and VEGF positive cells and IHS in experimental group were significantly higher than in control group (P ＜ 0. 05). Conclusion The expression of PDECGF/TP and VEGF was up-regulated in patients with IC. IC can be screened out or diagnosed by detecting PDECGF/TP and VEGF.

Application of PDECGF/TP and VEGF in the diagnosis of interstitial cystitis

Objective To find out the relationship among the expression of vascular growth factors in patients with interstitial cystitis (IC) and their performance under the cystoscopy as well as the clinical symptoms by detecting the expression of platelet-derived endothelial growth factor/thymine phosphatase (PDECGF/TP) and vascular endothelial growth factor (VEGF). Methods Twelve cases of IC served as the experimental group and 12 cases of type Ⅲ as the control group. The patients in experimental group had bladder mucosa biopsy. The number of mast cells was observed by HE staining. The expression of PDECGF/TP and VEGF and immunohistological score (IHS) were detected by using immunohistochemistry. Results Bladder mucosa hemorrhage points and sheets were observed under water expansion. The number of mast cells existing in the submucosa of the bladder tissue was significantly greater than in control group (P ＜ 0. 05 ). The percentage of PDECGF/TP and VEGF positive cells and IHS in experimental group were significantly higher than in control group (P ＜ 0. 05). Conclusion The expression of PDECGF/TP and VEGF was up-regulated in patients with IC. IC can be screened out or diagnosed by detecting PDECGF/TP and VEGF.