正常淋巴液对内毒素休克大鼠肺组织髓过氧化物酶活性及膜泵的作用研究

目的观察外源性正常淋巴液对脂多糖（LPS）所致内毒素休克大鼠肺组织匀浆髓过氧化物酶（MPO）活性及肺组织细胞膜泵功能的作用，探讨其干预机制。方法雄性Wistar大鼠40只，按随机数字表法均分为内毒素组、淋巴液组、血浆组和对照组。除对照组外，其他各组应用LPS5mg／kg复制内毒素休克模型，对照组以等量生理盐水代替LPS；15min后，淋巴液组自颈静脉输入仅占全血量1／15的无细胞淋巴液；血浆组以血浆代替淋巴液；内毒素组和对照组以生理盐水代替淋巴液。给予LPS（或相应液体）后4h，制备体积分数为10％的肺组织匀浆，检测肺组织匀浆MPO及膜ATP酶的活性。结果与对照组相比，内毒素组和血浆组大鼠肺组织匀浆MPO活性显著增高、4种膜ATP酶活性均显著下降（P＜0．05或P＜0．01）。淋巴液组肺组织匀浆MPO活性显著高于对照组，Na^＋-K^--ATP酶活性显著低于对照组，两组比较差异均有显著性（P均＜0．05）；而Ca^2＋-ATP酶、Mg^2＋-ATP酶和Ca^2＋-Mg^2＋-ATP酶活性与对照组比较差异均无显著性（P均＞0．05）；淋巴液组肺组织匀浆MPO活性显著低于内毒素组及血浆组（P均＜0．01），4种膜ATP酶活性显著高于内毒素组及血浆组（P＜0．05或P＜0．01）。结论外源性正常淋巴液对内毒素休克所致的肺损伤具有干预作用，其机制可能与减少中性粒细胞激活、提升膜ATP酶活性有关。

Effects of normal lymph on myeloperoxidase activity and adenosine triphosphatase in rats with endotoxic shock

Objective To observe the effects of exogenous normal lymph on myeloperoxidase (MPO) and adenosine triphosphatase (ATPase) activities of lung homogenate in rats with endotoxic shock caused by lipopolysaccharide (LPS), and to preliminarily discuss its mechanisms. Methods Forty male Wistar rats were randomly divided into four groups: endotoxin group, lymph group, plasma group and control group. The rats in the preceding three groups were injected intravenously with LPS (5 mg/kg. bw, iv) to replicate endotoxic shock model. LPS was replaced by equal volume of normal saline in the control group. Fifteen minutes later, lymph without cell components was infused in lymph group. The amount of lymph was 1/15 of blood volume. In plasma group, lymph was replaced by plasma, and in normal and endotoxin groups, lymph were replaced by normal saline. Four hours after the infusion of LPS, lung homogenate in a concentration of 10% was prepared. The activities of MPO and ATPase were determined in lung homogenate . Results Compared with control group, the activities of MPO in lung homogenate of endotoxin group and plasma group were significantly increased while the activities of ATPase were significantly lower (P<0.05 or P<0.01). The activity of MPO in lung homogenate of lymph group was significantly higher, while Na~+-K~+-ATPase activity was significantly lower than those of control group (both P<0.05). The activities of Ca~ 2+ -ATPase, Mg~ 2+ -ATPase and Ca~ 2+ -Mg~ 2+ -ATPase in lymph group showed no statistically significant differences compared with those of control group (all P>0.05), but when compared with the endotoxin group and plasma group, the activity of MPO in lung homogenate was significantly lower (both P<0.01) and the activities of 4 kinds of ATPase were significantly higher (P<0.05 or P<0.01). Conclusion The results demonstrate that exogenous normal lymph could ameliorate the lung injury as a result of endotoxic shock, and its mechanism might relate to reduction of activity of the polymorphonuclear leucocyte (PMN) and enhancement of the activity of ATPase.