蛋白酶活化受体1介导人胚肺成纤维细胞释放单核细胞趋化蛋白-1

目的：研究蛋白酶活化受体1（PAR1）对凝血酶诱导人胚肺成纤维细胞（HFL-1）释放单核细胞趋化蛋白-1（MCP-1）的调节作用。方法：HFL-1用PAR1激动剂凝血酶、PAR1活性肽SFLLR和反活性肽RLLFS进行诱导,并用PAR1阻断剂SCH79797进行阻断后用凝血酶和SFLLR刺激;用ELISA检测MCP-1浓度。结果：凝血酶及SFLLR可引起HFL-1释放MCP-1,而RLLFS不能引起MCP-1的释放增加;SCH79797可阻断凝血酶及SFLLR诱导HFL-1释放MCP-1。结论：PAR1介导凝血酶诱导的HFL-1细胞释放MCP-1。

Proteinase-activated Receptor 1 Mediates Monocyte Chemoattractant Protein-1 Release in Human Fetal Lung Fibroblasts

Objective：To investigate the role of proteinase-activated receptor 1（PAR1）in thrombin-induced monocyte chemoattractant protein-1（MCP-1）release in human fetal lung fibloblast-1（HFL-1）.Methods：HFL-1 was cultured in a 96-well culture plate.Cells were stimulated with thrombin,PAR1 agonist peptide SFLLR and its reverse peptide RLLFS or HFL-1 was pre-incubated with PAR1 antagonist SCH79797 before stimulation with thrombin or SFLLR.MCP-1 protein levels in supernatants were assessed by ELISA.Results：Both thrombin and SFLLR could induce HFL-1 MCP-1 release,but not RLLFS.SCH79797 could inhibit thrombin-and SFLLR-induced HFL-1 MCP-1 release.Conclusion：PAR1 mediates thrombin-induced MCP-1 release in cultured HFL-1.