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| 胃癌细胞中SerpinB5与MAFbx的相互作用及其作用位点研究 | |
| 引用本文： | 雷科锋,王翼飞,王群群,何香红,刘炳亚,陈雪华,俞焙秦,朱正纲.胃癌细胞中SerpinB5与MAFbx的相互作用及其作用位点研究[J].中华胃肠外科杂志,2012,15(2):169-173. |
| 作者姓名： | 雷科锋 王翼飞 王群群 何香红 刘炳亚 陈雪华 俞焙秦 朱正纲 |
| 作者单位： | 1. 浙江省人民医院普通外科 2. 上海大学理学院数学系 3. 200025,上海交通大学医学院附属瑞金医院外科上海消化外科研究所 |
| 基金项目： | 国家高技术研究发展计划("863"计划),国家自然科学基金,上海市重点基础研究 |
| 摘 要： | 目的 探讨胃癌细胞中SerpinB5能与MAFbx的相互作用及其作用位点.方法 采用酵母双杂交及免疫共沉淀方法检测SerpinB5与MAFbx之间的相互作用.分别用RNA干扰及瞬间转染pGBKT7-SerpinB5调节SerpinB5表达水平.在胃癌细胞株SUN-16中研究SerpinB5对MAFbx表达的影响.用同源建模的方法构建出MAFbx的结构信息;并以分子对接的手段来预测SerpinB5与MAFbx相互作用的位点.结果 酵母双杂交及免疫共沉淀均证实SerpinB5能与MAFbx相互作用;MAFbx的表达水平随着SerpinB5的表达水平变化而变化.同源建模和分子对接的方法预测SerpinB5与MAFbx蛋白相互作用的关键残基是PR0261、ASN361和LYS362.结论 胃癌细胞中SerpinB5能与MAFbx相互作用,且SerpinB5上的PR0261、ASN361、LYS362残基是其与MAFbx蛋白相互作用的可能位点. |
| 关 键 词： | 胃肿瘤 SerpinB5 MAFbx 酵母双杂交 计算分子生物学 |
Interaction of SerpinB5 and MAFbx in gastric cancer cell and its action site |
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| LEI Ke-feng , WANG Yi-fei , WANG Qun-qun , HE Xiang-hong , LIU Bing-ya , CHEN Xue-hua , YU Bei-qin , ZHU Zheng-gang.Interaction of SerpinB5 and MAFbx in gastric cancer cell and its action site[J].Chinese Journal of Gastrointestinal Surgery,2012,15(2):169-173. | |
| Authors: | LEI Ke-feng WANG Yi-fei WANG Qun-qun HE Xiang-hong LIU Bing-ya CHEN Xue-hua YU Bei-qin ZHU Zheng-gang |
| Institution: | Department of Surgery, Shanghai Jiaotong University School of Medicine, Shanghai, China. |
| Abstract: | Objective To explore the interaction between SerpinB5 and MAFbx in gastric cancer cell and to identify the interaction sites.Methods The interaction between SerpinB5 and MAFbx was screened and validated by yeast two-hybrid screening and co-immunoprecipitation.The expression of MAFbx was analyzed after SerpinB5 expression being modified by RNA interference and pGBKT7-SerpinB5 transfection.The impact of SerpinB5 on the expression of MAFbx was studied in gastric cancer cell line SUN-16.A model of MAFbx was constructed by homology modeling.The related residues for interaction were analyzed by Autodock4.0.Results The interaction between SerpinB5 and MAFbx was validated.The expression of MAFbx changed along with SerpinB5 expression.Amino acids including PRO261,ASN361,and LYS362 were key residue in the interaction of SerpinB5 and MAFbx.Conclusion SerpinB5 interacts with MAFbx in gastric cancer cell.Amino acids including PRO261,ASN361,and LYS362 are potential binding sites. |
| Keywords: | Stomach neoplasms SerpinB5 MAFbx Yeast two-hybrid Computational molecular biology |
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