急慢性乙型肝炎患者血浆uPA和uPAR的检测及意义

目的研究急慢性乙型肝炎uPA和uPAR的表达，探讨肝炎发病时血液纤溶的变化及意义。方法应用酶联免疫吸附试验（ELISA）测定血浆uPA和uPAR的水平。结果急慢性乙型肝炎血浆uPA和uPAR水平与对照组比较均有意义地高于对照组（P〈0．01）；慢性乙型肝炎重度组血浆uPA和uPAR水平显著高于急性乙型肝炎组（P〈0．05），亦显著高于慢性乙型肝炎中轻度组（P〈0．05和P〈0．01）；急性乙型肝炎血浆中uPAR水平显著高于慢性乙型肝炎中轻度组（P〈0．01）；乙型肝炎急性期血浆中uPA和uPAR水平显著升高，恢复期明显回落（P〈0．05和P〈0．01），但仍明显高于正常对照组（P〈0．01）；急慢性乙型肝炎血浆中uPAR水平与凝血酶原时间（PT）（r=0．605，P〈0．01）和国际标准化比率INR（r=0．603，P〈0．01）、胆红素（TB）（r=0．649P〈0．01）呈正相关。结论急慢性乙型肝炎uPA和uPAR水平的升高，与炎症的严重程度有关，与肝细胞损伤程度有关，是肝炎发病时血液凝血和纤溶系统失衡的重要原因之一。

Detection of plasma urokinase-type plasminogen activator and its receptor in acute or chronic hepatitis B patients and its clinical significance

Objective To study the expression of plasma uPA and uPAR in Acute or Chronic Hepatitis B patients,to investigate changes of blood fibrinolytic system in Hepatitis B and its clinical significance.Methods The level of detected plasma urokinase-type plasminogen activator(uPA) and urokinase-type plasminogen activator receptor(uPAR) were measured by Enzyme Linked Immuno Sorbent Assay (ELISA).Results The levels of plasma uPA and uPAR were significantly higher in patients with Acute or Chronic Hepatitis B than that of control group(P<0.05,P<0.01),and also higher in serious group than that of non-serious group of Chronic Hepatitis B patients (P<0.05),and also higher in chronic group than in acute group(P<0.05). The level of plasma uPAR was significantly higher in patients with serious jaundice than that of non-serious jaundice group(P<0.01). The levels of uPA and uPAR are significane higher in acute period than that in recover period of Acute Hepatitis B patients,but the levels in recover period is also higher than that in control group.The level of plasma uPAR have positive correlation with prothrombin(PT), international normalized ratio(INR) and total bilirubin(P<0.01). Conclusion The level of plasma UPA nad UPAR were enhanced in Acute or Chronic Hepatitis B patients was related with the degree of inflammation,was also related with high expression of serious damage of liver cell.It may be one of important cause to inbalance between coagulation and fibrinolytic in hepatitis.