蛋白质组技术在胃癌相关标志物筛查中的应用

目的 建立胃癌组织和正常胃组织双向凝胶电泳图谱，鉴定差异表达蛋白，从中发现有意义的胃癌相关标志物。方法 采用双向凝胶电泳（2-DE）分离胃癌组织和正常胃组织总蛋白，银染显色，PDQuest软件分析，从中选取差异表达蛋白质点，通过基质辅助激光解析电离飞行时间质谱（MALDI-TOF-MS）或MALDI-TOF-TOF-MS鉴定差异表达的蛋白质。结果 获得重复性和分辨率较好的胃组织双向凝胶电泳图谱；发现23个差异表达蛋白质，其中15个得到鉴定，在肿瘤组织中高表达的有10个，其余5个在肿瘤组织中低表达。结论 建立了胃癌组织和正常胃组织双向凝胶电泳图谱，并应用质谱技术鉴定了15个差异表达蛋白质。

Application of technologies of Proteome for screening molecular biomarkers associated with gastric cancer

Objective To establish two-dimensional electrophoresis(2-DE)profiles of human gastric cancer tissue and paired normal tissue,and to identify differential expression proteins,in which try to find out candidates as gastric cancer-related proteins.Methods 2-DE was applied to separate the total proteins of gastric cancer tissue and paired normal tissue.After staining and analyzing by PDQuest soft- ware,the differential expression proteins were identified by matrix-assisted laser desorption/ionization- time of flight mass spectrometry(MALDI-TOF-MS)or MALDI-TOF-TOF-MS.Results 2-DE profiles with high resolution and reproducibility were obtained.Twenty-three protein spots were found as differ- entially-expressed proteins,in which 15 protein spots were identified successfully.Among the identified proteins,there were 10 over-expressed and 5 under-expressed proteins in gastric cancer tissue as compared with normal tissue.Conclusion The well-resolved,reproducible 2-DE patterns of human gastric cancer tissue and paired normal tissue were established and fifteen differentially expressed proteins were identi- fied.