重组人胶质细胞源性神经营养因子对大鼠周围神经再生的作用

目的：研究胶质细胞源性神经营养因子（glial cell line-derived neurotrophic factor,GDNF)对周围神经再生的作用，方法：硅胶管套接大鼠切断了的坐骨神经，术中一次性将GDNF、生理盐水（SAL）分别加入硅胶管中，术后4周，应用溃疡神经纤维染色法、辣根过氧化物酶（horseradish peroxidase,HRP)逆行追踪技术观察GDNF对轴突再生的影响。结果：与SAL组相比，GDNF组溃变神经纤维面积明显减少，SAL组溃变纤维面积百分率为17．3％，GDNF组为1．9％（P＜0．01）；GDNFXEG组脊髓HRP标记胞体显著增加，SAL组标记胞体数的百分率为43．5％，GDNF组为68．3％（P＜0．01）。结论：外源性GDNF能明显促进周围神经再生。

Effect of recombinant human glial cell line-derived neurotrophic factor (GDNF) on peripheral nerve regeneration in rats

Objective: To study the effect of GDNF on peripheral nerve regeneration. Methods: Transected sciatic nerve in rat was sutured into silicone chamber. GDNF or SAL solution was injected into the silicone chambers during operation. Four weeks later, the effect of GDNF on axonal regeneration was evaluated by degenerative neurofiber staining and HRP retrograde tracing. Results: Compared with SAL group, the percentage of degenerative neurofibers areas was decreased from 17.3% in SAL group to 1.9% in GDNF group( P <0.01)and the percentage of labeled spinal somas number was significantly increased from 43.5% in SAL group to 68.3% in GDNF group( P <0.01). Conclusion: The results suggest that exogenous GDNF can obviously enhance peripheral nerve regeneration.