| 2006年长春市儿童野生型麻疹病毒基因序列分析 |
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| 引用本文： | 胡钰,刘国梁,李凡.2006年长春市儿童野生型麻疹病毒基因序列分析[J].中国妇幼保健,2008,23(10):1407-1409. |
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| 作者姓名： | 胡钰 刘国梁 李凡 |
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| 作者单位： | 吉林大学白求恩医学院病原生物学教研室,吉林,长春,130021 |
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| 摘 要： | 目的:研究吉林省长春市0~12周岁儿童野生型麻疹病毒的基因型别及核蛋白(N)基因序列特征,探讨控制麻疹发生的措施。方法:采用Vero/SLAM细胞分离培养麻疹病毒,通过逆转录-聚合酶链反应(RT-PCR)方法从分离的麻疹病毒中扩增核蛋白(N)基因羧基端450个核苷酸片段(bp)并进行核苷酸测序分析,对基因库中麻疹病毒的22个代表株基因序列的同源性进行比较。结果:分离得到9株野生型麻疹病毒,N基因与H1基因型代表株相比同源性为98.3%。结论:吉林省长春市麻疹流行株属于H1基因型。
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| 关 键 词： | 麻疹病毒 核蛋白N 序列分析 |
| 文章编号： | 1001-4411(2008)10-1407-03 |
| 修稿时间： | 2007年4月25日 |
Sequence analysis on nucleoprotein gene of wild-type measles virus isolated at changchun Jilin province in 2006. |
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| HU Yu,LIU Guo-Liang,LI Fan.Sequence analysis on nucleoprotein gene of wild-type measles virus isolated at changchun Jilin province in 2006.[J].Maternal and Child Health Care of China,2008,23(10):1407-1409. |
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| Authors: | HU Yu LIU Guo-Liang LI Fan |
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| Abstract: | Objective:To study the genetic characteristics and genotype of measles strains and the measures to control measles in Jilin province.Methods:Throat swabs were collected for virus isolation with Vero/SLAM cells.450 nucleotides fragment of the C-terminal of the nucleoprotein (N) gene were amplified by RT-PCR and subjected to sequence and phylogenetic analysis using DNAstar software.Results:Nine strains were isolated, N genes homogeneity was 98.3% compared with other wild-type viruses of Hl genotype.Conclusion:The circulating measles viruses are belong to clade H1 in Jilin province. |
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| Keywords: | Measles virus Nucleoprotein gene Sequence analysis |
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