| STUDY ON EFFECTS OF QUERCETIN ON PML GENE AND PROTEIN IN LEUKEMIA CELL LINES |
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| 作者姓名： | 钟璐 陈芳源 韩洁英 邵念贤 欧阳仁荣 |
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| 作者单位： | ZHONG Lu，CHEN Fang-yuan，HAN Jie-ying，SHAO Nian-xianOUYANG Ren-rongDepartment of Leukemia Research，Shanghai Institute of Hemutology，Renji Hospital，SSMU，Shanghai 200001，China |
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| 摘 要： | Objective To investigate the effect of quercetin on PML gene and protein expression andlocalization in leukemia cell lines. Methods Cell morphology was assayed by Wright's stain and fluorescence stain, and PML mRNA expression by RT-PCR , PML protein localization by immuno-fluorescence. Results NB4 and HL-60 cells differentiated morphologically after treatment with all-trans-retinoic acid (ATRA) while K562 cells did not differentiate. Typical apoptosis was found in each cell line after treatment with quercetin. Immuno-fluorescence analysis showed , after treatment with ATRA , the fusion protein disappeared in NB4 cells and PML protein relocated , while HL-60 and K562 cells had no difference from control cells. After treatment with quercetin, the fusion protein disappeared in NB4 cells, PML protein relocated, then degraded. In HL-60 cells and K562 cells, PML protein also located and then degraded . The expression of PML mRNA was not changed in all three cell lines after treatment with ATRA or quercetin. C
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STUDY ON EFFECTS OF QUERCETIN ON PML GENE AND PROTEIN IN LEUKEMIA CELL LINES |
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| ZHONG Lu,CHEN Fang-yuan,HAN Jie-ying,SHAO Nian-xianOUYANG Ren-rong.STUDY ON EFFECTS OF QUERCETIN ON PML GENE AND PROTEIN IN LEUKEMIA CELL LINES[J].Journal of Shanghai Second Medical University(Foreign Language Edition),2003,15(1). |
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| Authors: | ZHONG Lu CHEN Fang-yuan HAN Jie-ying SHAO Nian-xianOUYANG Ren-rong |
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| Institution: | ZHONG Lu,CHEN Fang-yuan,HAN Jie-ying,SHAO Nian-xianOUYANG Ren-rongDepartment of Leukemia Research,Shanghai Institute of Hemutology,Renji Hospital,SSMU,Shanghai 200001,China |
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| Abstract: | Objective To investigate the effect of quercetin on PML gene and protein expression andlocalization in leukemia cell lines. Methods Cell morphology was assayed by Wright's stain and fluorescence stain, and PML mRNA expression by RT-PCR , PML protein localization by immuno-fluorescence. Results NB4 and HL-60 cells differentiated morphologically after treatment with all-trans-retinoic acid (ATRA) while K562 cells did not differentiate. Typical apoptosis was found in each cell line after treatment with quercetin. Immuno-fluorescence analysis showed , after treatment with ATRA , the fusion protein disappeared in NB4 cells and PML protein relocated , while HL-60 and K562 cells had no difference from control cells. After treatment with quercetin, the fusion protein disappeared in NB4 cells, PML protein relocated, then degraded. In HL-60 cells and K562 cells, PML protein also located and then degraded . The expression of PML mRNA was not changed in all three cell lines after treatment with ATRA or quercetin. Conclusion PML plays the role of differentiation and apoptosis inducer in leukemia cells at the translational level. PML in POD plays the role of apoptosis inducer and the growth control of leukemia cells. |
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| Keywords: | quercetin ATRA PML NB4 cell HL-60 cell K562 cell |
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