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| 新生兔雪旺细胞体外培养及纯化的实验研究 | |
| 引用本文： | 马立,刘彦普,赵丽君. 新生兔雪旺细胞体外培养及纯化的实验研究[J]. 中国组织工程研究与临床康复, 2002, 6(20): 3036-3037 |
| 作者姓名： | 马立 刘彦普 赵丽君 |
| 作者单位： | 解放军第四军医大学口腔医院,陕西 西安 710032 |
| 摘 要： | 目的优化雪旺细胞(SCs)的体外培养条件,在去除成纤维细胞(FCs)沾染后,应用碱性成纤维细胞生长因子(bFGF)促进SCs的增殖以获取大量、纯净SCs应用于神经组织工程。方法采用高浓度胶原酶分次消化分离SCs,使用阿糖胞苷、Geneticin去除FCs,待基本为纯净的SCs后,再用bFGF促进其分裂增殖。结果经S-100染色鉴定,SCs纯度可达到98%以上。结论文章所用的方法是培养和促进SCs增殖较为理想的方法。 |
| 关 键 词： | 雪旺细胞 bFGF 细胞培养 组织工程 |
| 文章编号： | 1671-5926(2002)20-3036-02 |
| 修稿时间： | 2002-06-15 |
Experimental study of culturing and purifying Schwann cells of neonatal rabbits |
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| MA Li,LIU Yan-pu,ZHAO Li-jun.Stoma tological Hospital,Fourth Military Medical U niversity,Xi ' an ,China. Experimental study of culturing and purifying Schwann cells of neonatal rabbits[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2002, 6(20): 3036-3037 | |
| Authors: | MA Li LIU Yan-pu ZHAO Li-jun.Stoma tological Hospital Fourth Military Medical U niversity Xi ' an China |
| Affiliation: | MA Li,LIU Yan-pu,ZHAO Li-jun.Stoma tological Hospital,Fourth Military Medical U niversity,Xi ' an 710032,China |
| Abstract: | Objective Through eliminating fibroblast and promoting proliferation of Schwann cells(SCs )we optimize culture condition of SCs in vitro in order to obtain more population and more purification of SC s to repair peripheral nerve.Methods High-density collagenase was used to separate SCs.Cytosine arabi noside and Geneticin were used to eli minate fibroblast.Then bFGF was add ed to promote proliferation of SCs.Results It was manifested that purification of SCs can attain above 98%through th e result of rabbit anti-S-100stain.Conclusion Method used in our experiment is an id eal approach to obtain purified and l arge quantity of SCs. |
| Keywords: | Schwann cells bFGF cell culture tissue engineering |
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