| 高与低表达RSK4基因人乳腺癌细胞株的筛选及鉴定 |
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| 引用本文： | 张晓丽,刘剑仑,杨华伟,韦薇,蒋奕. 高与低表达RSK4基因人乳腺癌细胞株的筛选及鉴定[J]. 中华肿瘤防治杂志, 2011, 18(8): 561-563,574 |
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| 作者姓名： | 张晓丽 刘剑仑 杨华伟 韦薇 蒋奕 |
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| 作者单位： | 广西医科大学附属肿瘤医院乳腺外科,广西南宁,530021 |
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| 基金项目： | 国家自然科学基金(30960427) |
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| 摘 要： | 目的:探讨核糖体S6激酶4(RSK4)抑癌基因在人乳腺癌细胞株MDA-MB-231、T47D、Bcap37和MCF-7中的表达,并筛选出高和低表达RSK4基因的乳腺癌细胞。方法:逆转录聚合酶链反应(RT-PCR)和蛋白质印迹法从mRNA和蛋白水平检测RSK4在MDA-MB-231、T47D、Bcap-37和MCF-7 4种乳腺癌细胞株中的表达。结果:人乳腺癌细胞株MDA-MB-231、T47D、Bcap37和MCF-7中均有RSK4基因表达,其中,MCF-7细胞RSK4表达水平最高,为表达量最低的MDA-MB-231细胞的2.9倍,MCF-7细胞RSK4蛋白表达水平也最高,与其他3种细胞株相比,差异有统计学意义,P<0.001。RT-PCR法和蛋白质印迹法检测结果一致,具有高度相关性,r=0.766,P<0.001。结论:人乳腺癌细胞株MCF-7为高表达RSK4基因的细胞,MDA-MB-231细胞为低表达RSK4基因的细胞,两者均可作为人乳腺癌细胞中研究RSK4基因的实验材料。
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| 关 键 词： | 乳腺肿瘤 细胞系,肿瘤 核糖体蛋白质S6激酶类 基因表达 |
Screening of high and low expressed RSK4 genehuman breast cancer cell lines |
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| ZHANG Xiao-li,LIU Jian-lun,YANG Hua-wei,WEI Wei,JIANG Yi. Screening of high and low expressed RSK4 genehuman breast cancer cell lines[J]. Chinese Journal of Cancer Prevention and Treatment, 2011, 18(8): 561-563,574 |
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| Authors: | ZHANG Xiao-li LIU Jian-lun YANG Hua-wei WEI Wei JIANG Yi |
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| Affiliation: | ZHANG Xiao-li,LIU Jian-lun,YANG Hua-wei,WEI Wei,JIANG YiDepartment of Breast Diseases Surgery,Affiliated Tumor Hospital,Guangxi Medical University,Nanning 530021,P.R.China |
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| Abstract: | OBJECTIVE:To detect the RSK4 gene expression in MDA-MB-231,T47D,Bcap37,MCF-7 human breast cancer cell lines,and select the highest and lowest expression of RSK4 cell lines for the further study.METHODS:RT-PCR and Western-blot were used to verify the mRNA and protein levels of expression of RSK4.RESULTS:RSK4 gene expressed in all four human breast cancer cell lines,and the expression of RSK4 mRNA in MCF-7 was the most highest,which was 2.9 times higher than the lowest-expressed MDA-MB-231 cell line;meanwhile... |
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| Keywords: | breast neoplasms cell line tumor ribosomal protein S6 kinases gene expression |
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