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| 曲古抑素A对耐顺铂人肺腺癌细胞株凋亡影响及其机制的探讨 | |
| 引用本文： | 伍俊,胡成平. 曲古抑素A对耐顺铂人肺腺癌细胞株凋亡影响及其机制的探讨[J]. 中华肿瘤防治杂志, 2011, 18(13) |
| 作者姓名： | 伍俊 胡成平 |
| 作者单位： | 1. 中南大学湘雅医院呼吸内科，湖南长沙410008;广东医学院呼吸疾病研究所，广东湛江524023 2. 中南大学湘雅医院呼吸内科,湖南长沙,410008 |
| 摘 要： | 目的:探讨曲古抑素A(TSA)诱导耐顺铂(DDP)人肺腺癌A549/DDP细胞株凋亡的作用及机制。方法:荧光染色检测细胞凋亡,蛋白质印迹法分析cFLIP、Caspase-8、Caspase-3和聚腺苷二磷酸核糖聚合酶(PARP)蛋白表达的变化,同时比色法测定Caspase-8活性。结果:曲古抑素A能诱导凋亡A549/DDP细胞株凋亡,随着浓度增加,其凋亡率逐渐增加。蛋白质印迹法结果显示,TSA处理细胞后,cFLIP蛋白水平下降,Pro-caspase-8减少,提示该酶被激活,促使Bid蛋白切割。Procaspase-3减少,提示酶被激活。PARP蛋白减少,切割的条带逐渐增加,提示PARP活性增加,促进细胞凋亡。比色法结果显示TSA对Caspase-8活性和cFLIP的影响具有浓度和时间依赖性。结论:TSA可以通过降低cFLIP水平激活Caspase-8诱导A549/DDP细胞凋亡。 |
| 关 键 词： | 曲古抑素A A549/DDP cFLIP 细胞凋亡 Caspase-8 Bid |
Mechanism and effect of trichostatin A on apoptosis of cisplatin-resistant human lung adenocarcinoma cell line |
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| WU Jun,HU Cheng-ping. Mechanism and effect of trichostatin A on apoptosis of cisplatin-resistant human lung adenocarcinoma cell line[J]. Chinese Journal of Cancer Prevention and Treatment, 2011, 18(13) | |
| Authors: | WU Jun HU Cheng-ping |
| Affiliation: | WU Jun1,2,HU Cheng-ping1 1.Department of Respiratory Medicine,Xiangya Hospital,Central South University,Changsha 410008,P.R.China2.Institute of Respiration,Guangdong Medical College,Zhanjiang 524023,P.R.China |
| Abstract: | OBJECTIVE: To investigate the mechanism of Trichostatin A(TSA) induced apoptosis of cisplatin-resistant human lung adenocarcinoma cell line A549/DDP.METHODS: Morphologic assessment of apoptosis was performed with fluorescence microscope;Different proteins such as cFLIP,Caspase-8,Caspase-3,and PAPP were analyzed by Western blotting method,and Colorimetric method was used to measure Caspase-8 activities.REULTS: TSA induced apoptosis in cisplatin-resistant A549/DDP cells in dose-dependent manner,the apoptotic ... |
| Keywords: | TSA A549/DDP cFLIP apoptosis Caspase-8 Bid |
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