| 肾癌细胞增殖过程MACC1和c-MET调控机制的探讨 |
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| 引用本文： | 尚超,张辉,宋永胜.肾癌细胞增殖过程MACC1和c-MET调控机制的探讨[J].中华肿瘤防治杂志,2011,18(14):1065-1067,1087. |
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| 作者姓名： | 尚超 张辉 宋永胜 |
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| 作者单位： | 1. 中国医科大学基础医学院神经生物教研室,辽宁沈阳,110001
2. 中国医科大学附属盛京医院泌尿外科,辽宁沈阳,110004 |
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| 基金项目： | 国家自然科学基金(30901480) |
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| 摘 要： | 目的:探讨MACC1和c-MET与肾癌的相关性,明确两者之间调控关系及对肾癌Caki-1细胞增殖的影响。方法:应用荧光定量PCR检测67例肾癌及相应癌旁正常组织中MACC1和c-MET mRNA的表达。设计并合成MACC1基因特异性的siRNA,转染肾癌Caki-1细胞,检测转染后MACC1和c-MET蛋白的表达以及Ca-ki-1细胞的细胞抑制率。结果:肾癌组织中的MACC1和c-MET mRNA表达较癌旁正常组织中均有明显增加(P<0.05),且两者呈正相关。siRNA-MACC1能够明显抑制Caki-1细胞中MACC1蛋白的表达,P<0.05。MACC1蛋白表达抑制的Caki-1细胞中c-MET蛋白的表达亦降低(P<0.05),细胞抑制率明显升高,P<0.05。结论:MACC1和c-MET都与肾癌相关,MACC1能够调节下游基因c-MET的表达,影响肾癌细胞的增殖能力,发挥促进肿瘤生长的作用。
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| 关 键 词： | 肾肿瘤 MACC1 肝细胞生长因子受体 细胞增殖 |
Regulation between MACC1 and c-MET in proliferative process of renal carcinoma |
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| SHANG Chao,ZHANG Hui,SONG Yong-sheng.Regulation between MACC1 and c-MET in proliferative process of renal carcinoma[J].Chinese Journal of Cancer Prevention and Treatment,2011,18(14):1065-1067,1087. |
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| Authors: | SHANG Chao ZHANG Hui SONG Yong-sheng |
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| Institution: | SHANG Chao1,ZHANG Hui2,SONG Yong-sheng21.Department of Neurobiology,College of Basic Medical Science,China Medical University,Shenyang 110001,P.R.China2.Department of Urinary Surgery,Affiliated Shengjing Hospital,Shenyang 110004,P.R.China |
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| Abstract: | OBJECTIVE: To research the relationship of MACC1 and c-MET in renal carcinoma,and their influence to proliferation of Caki-1 cells.METHODS: Fluorescent quantitation PCR was used to detect the MACC1 and c-MET mRNA in 67 cases of renal carcinoma and peri-cancerous normal tissues.Specific siRNA for MACC1 gene was designed and synthesized,and then Caki-1 cells were transfected with siRNA-MACC1 to inhibit the expression of MACC1.After RNA interference,the expression of c-MET and the proliferation of Caki-1 cells... |
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| Keywords: | renal neoplasms MACC1 c-MET cell proliferation |
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