| 高效液相色谱法测定金钗石斛中石斛碱含量 |
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| 引用本文： | 李墅,王春兰,郭顺星. 高效液相色谱法测定金钗石斛中石斛碱含量[J]. 中国药学杂志, 2009, 44(4): 252-254 |
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| 作者姓名： | 李墅 王春兰 郭顺星 |
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| 作者单位： | 中国医学科学院-中国协和医科大学药用植物研究所 北京 100094 |
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| 基金项目： | 国家自然科学基金资助项目(30770068) |
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| 摘 要： | 目的建立一种用HPLC测定石斛中石斛碱含量的方法。方法Waters XTerraTM RP18色谱柱(3.9mm×150mm,5μm),流动相为乙腈-水-三乙胺(21∶79∶0.005),流速为1mL·min-1,检测波长为210nm。结果石斛碱在30.9～618mg·L-1内与峰面积呈良好的线性关系,回归方程为Y=3191.5ρ+14191(r=0.9999),平均回收率为99.11%,RSD为2.60%。结论应用HPLC测定石斛中石斛碱含量的方法简便、准确、可行。
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| 关 键 词： | 金钗石斛 高效液相色谱法 石斛碱 含量测定 |
| 收稿时间： | 2008-03-29; |
Determination of Dendrobin in Dendrobium nobile by HPLC Analysis |
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| LI Shu,WANG Chun-lan,GUO Shun-xing. Determination of Dendrobin in Dendrobium nobile by HPLC Analysis[J]. Chinese Pharmaceutical Journal, 2009, 44(4): 252-254 |
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| Authors: | LI Shu WANG Chun-lan GUO Shun-xing |
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| Affiliation: | Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100094,China |
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| Abstract: | OBJECTIVE To establish a HPLC method for the determination of dendrobin in Dendrobium nobile. METHODS The determination was carried out on a Waters XTerraTM RP18(3.9 mm×150 mm,5 μm) column with acetonitril-water-triethylamine (21∶79∶0.005)as the mobile phase. The UV wavelength was 210 nm . RESULTS The calibration curve was in good linearity over the range of 30.9-618 mg·L-1 with the regression equation Y=3 191.5ρ+14 191 (r=0.999 9). The average recovery was 99.11% (RSD=2.61%). CONCLUSION The method was proved to be simple, accurate, reproducible, and feasible for determining the content of dendrobin in D. nobile. |
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| Keywords: | D.nobile.')" href=" #" >D.nobile. HPLC dendrobin determination |
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