制备薄层色谱 联合 FTIR 检测中药制剂中添加化学药品方法的研究

 目的 建立中药制剂中非法添加化学药品的检测方法。 方法 采用制备薄层色谱将掺有非法添加化学药品的中药制剂进行分离、浓缩后，测定其红外光谱图，将可疑成分的红外光谱图与其在薄层色谱上对应对照品的红外光谱图进行比较，根据它们的峰位置、峰形状和相对峰强度来判定中药制剂中是否非法添加了化学药品。 结果 用该方法对 3 种镇静安神类和 1 种止喘类中药制剂进行检测，我们检测出解郁安神颗粒中有非法添加的地西泮，丹莲安神胶囊中有非法添加的氯硝西泮，消喘灵中有非法添加的醋酸泼尼松和地西泮。 结论 将制备薄层色谱与红外光谱法结合起来，可快速准确地判定中药制剂中是否含有非法添加的化学药品，为中药制剂中非法掺入化学药品的检测提供了一种新的方法和手段。

Determination of Chemical Drugs Added to Traditional Chinese Medicine by Preparative TLC Coupled with FTIR

OBJECTIVE To develop a method to identify the chemical drugs added illegally to traditional Chinese medicine. METHODS Preparative TLC was used to separate and concentrate the chemical drugs added illegally to four traditional Chinese medicines. We scaned the fourier-transform infrared pectroscopy (FTIR)spectra was used to screen the doubtful components. Their similarities in the absorption frequency, peak shape and peak intensity with the reference IR spectra were compared to give the last conclusion. RESULTS Diazepam was added illegally in Jieyuanshen granules and not in Anshenbunao mixture,clonazepam was added illegally in Danliananshen Capsules ,prednisone acetate and diazepam were added illegally in Xiaochuanling. And this conclusion was the same as the result of the MS detection. CONCLUSION Preparative TLC coupled with FTIR could become a new approach to identify the chemical drugs added illegally to traditional Chinese medicine exactly.