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| 牛心细胞色素C氧化酶的纯化 | |
| 引用本文： | 赵建钢,杨同书. 牛心细胞色素C氧化酶的纯化[J]. 吉林大学学报(医学版), 1993, 0(5) |
| 作者姓名： | 赵建钢 杨同书 |
| 作者单位： | 中国康复研究中心(赵建钢)，白求恩医科大学应用基础医学研究所(杨同书) |
| 摘 要： | 本文应用硫酸铵分级沉淀、Phenyl-Sepharose CL-4B 疏水层析和 Cytocrome C-Sepharose 4B亲和层析的方法,从牛心肌中分离纯化了细胞色素C氧化酶。产品Heme a含量为16.32nmol/mg Pr,铜原子含量为15.24n atoms/ms Pr。活性实验表明,去脂的细胞色素 C氧化酶活性下降,加入Tween 80可使活性明显提高,说明疏水环境对酶的催化功能有保护作用。 |
| 关 键 词： | 细胞色素氧化酶/分离和提纯 疏水层析 亲和层析方法 酶激活 |
The purification of cytochrome C oxidase from bovine heart |
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| Zhao Jiangang,Yang Tongshu. The purification of cytochrome C oxidase from bovine heart[J]. Journal of Jilin University: Med Ed, 1993, 0(5) | |
| Authors: | Zhao Jiangang Yang Tongshu |
| Affiliation: | Institute of Preclinical Sciences |
| Abstract: | This paper reports the purification of CCO from bovine heart with ammonium sulfate precipitation, phneyl - Sepharose CL-4B hydrophobic chromatographic and cytochrome C-Sepharose 4B affinity chromatographic methods. The content of heme a and copper in the purified CCO reached 16. 3 n mols/mgPr and 15. 24 n atoms/mgPr. It was indicated that the activity of CCO decreased after removing the lipids. and rised by adding Tween 80. Hydrophobic environment of CCO activity site provided the protection to the catalytic function. |
| Keywords: | cytochrome oxidases/IP: chromatography affinity/MT: enzyme activation |
| 本文献已被 CNKI 等数据库收录！ | |