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| 多糖生物膜细胞载体特性的实验研究 | |
| 引用本文： | 耿燕,杨朝忠,刘万顺. 多糖生物膜细胞载体特性的实验研究[J]. 眼科研究, 2003, 21(5): 501-504 |
| 作者姓名： | 耿燕 杨朝忠 刘万顺 |
| 作者单位： | 1. 266042,青岛,青岛大学医学院第二附属医院眼科 2. 青岛东方眼科医院 3. 青岛海洋大学生命学院 |
| 摘 要： | 目的 探讨多糖生物膜(PSBM)作为角膜内皮细胞培养及移植载体的可行性。方法 通过PSBM的不同制剂给予大白鼠口服，悬浮培养仓鼠肾(BHK21)细胞及兔角膜层间植入，观察其毒性、生物相容性及可吸收性。结果 食用含多糖微颗粒的饲料60d后，大白鼠无肝肾功能及组织学改变；PSBM微载体悬浮培养72h后，细胞密度可增值3倍以上；角膜层间植入PSBM后2～3个月时，植人物基本吸收。结论 PSBM属安全无毒物质，具有很好的细胞亲和性、生物相容性及可吸收性，是CE细胞培养及移植的良好载体。 |
| 关 键 词： | 多糖生物膜 细胞载体 实验 角膜内皮细胞 细胞培养 毒性 生物相容性 可吸收性 |
| 修稿时间： | 2002-08-10 |
Experimental research on polysaccharide biomembrane as a carrier of corneal endothelial cells |
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| Geng Yan,Yang Chaozhong,Liu Wanshun. Experimental research on polysaccharide biomembrane as a carrier of corneal endothelial cells[J]. Chinese Ophthalmic Research, 2003, 21(5): 501-504 | |
| Authors: | Geng Yan Yang Chaozhong Liu Wanshun |
| Affiliation: | Geng Yan,Yang Chaozhong,Liu Wanshun. Department of Ophthalmology,Second Affiliated Hospital of Medical College,Qingdao University,Qingdao 266042 |
| Abstract: | Objective To study the possibility of polysaccharide biomembrane ( PSBM ) as a carrier in cultured and transplanted corneal endothelial ( CE) cells. Methods ( 1 ) BHK21 cells were cultured suspendly on the polysaccharide microcarrier in RPMI1640 medium supplemented with 10% fetal bovine serum. (2) The PSBM was placed in rabbit' s corneal stroma. Results (1) The concentration of HBK21 cells increased three-folds at 74 h after culture. (2) PSBM in corneal stroma induced slight neovascularization along the wound and disappeared gradually at 2-3 postoperative months. Conclusion PSBM seem to have better biocompatibility and may be an excellent carrier for CE cells' culture and transplantation. |
| Keywords: | polysaccharide biomembrane cell carrier endothelial cells/cornea |
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