支气管哮喘患者外周血中的CD+4 CD+25 T淋巴细胞的测定

目的阐明支气管哮喘(简称哮喘)患者外周血中是否存訡D+4 CD+25 T调节性淋巴细胞,并探讨CD+4 CD+25细胞的免疫抑制活性.方法应用流式细胞仪检测29例过敏性哮喘患者[急性发作期患者(急性发作期)15例、缓解期患者(缓解期)14例和16名非过敏性健康志愿者(正常对照组)外周血中CD+4 CD+25 细胞数量变化.应用免疫磁珠法分离提纯CD+4 CD+25 细胞,将纯化的CD+4 CD+25细胞和(或)CD+4 CD-25细胞在体外培养,观察CD+4 CD-25细胞的增殖反应,将收集培养的上清液用酶联免疫吸附测定(ELISA)法检测Th1类细胞因子γ干扰素(IFN-γ)和Th2类细胞因子白细胞介素4(IL-4)和IL-13.结果急性发作期患者外周血中CD+4 CD+25细胞比值为(14.9±1.8)%,缓解期患者为(11.8±0.7)%,正常对照组为(11.2±0.8)%,发作期与缓解期患者比较差异有统计学意义(P<0.01);缓解期患者与正常对照组比较差异无统计学意义(P>0.05).哮喘组CD+4 CD-25细胞增殖反应为(74±9)%,正常对照组为(72±8)%,两组比较差异无统计学意义(P>0.05).此外,哮喘组和正常对照组的CD+4 CD+25 细胞均能抑制CD+4 CD-25细胞产生IFN-γ、IL-4和IL-13,而且两组的抑制能力也无明显区别.结论急性发作期哮喘患者外周血CD+4 CD+25 细胞数明显高于缓解期患者和正常对照组.哮喘患者的CD+4 CD+25 细胞的抑制活性和正常对照组比较差异无统计学意义.谮

CD+4CD+25 T lymphocytes in peripheral blood from patients with asthma

OBJECTIVE: To explore whether regulatory CD(4)(+)CD(25)(+) cells exist in patients with atopic asthma. METHODS: The numbers of peripheral blood CD(4)(+)CD(25)(+) cells in peripheral blood of atopic asthmatics and healthy nonatopic subjects were determined using flow cytometry. CD(4)(+)CD(25)(+) and CD(4)(+)CD(25)(-) cells from atopic asthmatics and normal donors were isolated, and were cultured to observe the effects of CD(4)(+)CD(25)(+) cells on proliferation response as well as Th1/Th2 cytokine production of CD(4)(+)CD(25)(-) cells in vitro. RESULTS: A significant increase in CD(4)(+)CD(25)(+) cell numbers was shown in atopic asthmatic patients during acute exacerbation [(14.9 +/- 1.8)%, P < 0.01], but not in patients with stable asthma [(11.8 +/- 0.7)%] and normal subjects [(11.2 +/- 0.8)%, P > 0.05]. The mean inhibition values of the proliferation response of CD(4)(+)CD(25)(-) cells by CD(4)(+)CD(25)(+) cells from normal controls [(72 +/- 8)%] and asthmatics [(74 +/- 9)%] were similar (P > 0.05). There was no difference in inhibitory effects on both Th1 and Th2 cytokine production of CD(4)(+)CD(25)(-) cells by CD(4)(+)CD(25)(+) cells in the two groups. CONCLUSION: Although CD(4)(+)CD(25)(+) cells increase in atopic asthma during exacerbation, these regulatory T cells appear to function normally with regard to their suppressive activities.