舌鳞癌组织中血管内皮生长因子－D 的表达与淋巴管生成的关系及意义

目的：检测血管内皮生长因子－D（ VEGF－D）在舌鳞癌组织中的表达情况及舌鳞癌癌内和癌周淋巴管密度，并探讨VEGF－D与舌鳞癌淋巴管密度（ LVD）的关系及其临床意义。方法采用免疫组化染色法检测60例舌鳞癌组织（观察组）和12例正常舌黏膜组织（对照组）中VEGF－D和LVD的表达情况，统计分析VEGF－D与癌内、癌周LVD之间的相关性及临床意义。结果观察组的 VEGF －D高表达率为60.00％，对照组为25.00％，两组差异有统计学意义（ P＝0.026）。对照组的LVD高表达率为20％，观察组的癌周LVD高表达率为58.33％、癌内LVD高表达率为48.33％，与对照组比较，差异均有统计学意义（P＝0.008，0.043）。 VEGF－D和癌周LVD的高表达与淋巴结转移、临床分期、生存状态及预后之间有关（ P＜0.05）。癌内LVD的高表达仅与肿瘤T分类有关（P＝0.039）。 VEGF－D与癌周LVD在舌鳞癌中呈正相关（r＝0.966， P＜0.001）。结论 VEGF－D和淋巴管生成与肿瘤的侵袭、转移及预后密切相关，可作为指导舌鳞癌诊治及判断预后的重要指标。

Relationship between vascular endothelial growth factor-D expression and lymphangiogenesis in tongue squa-mous cell carcinoma and their clinical significance

Objective The purpose of this study was to detect vascular endothelial growth factor -D( VEGF-D) expression, peritumoral and intratumoral lymphatic vessel density ( LVD) in tongue squamous cell carcinoma ( TSCC), and to investigate the relationship between the expression of VEGF -D and LVD in TSCC and their clinical significance . Methods Expression of VEGF-D and LVD in 60 cases of TSCC and 12 cases of normal tongue tissue were detected by means of immunohistochemical staining .Correlation between peritumoral and intratumoral LVD and the expression of VEGF-D was analyzed , as well as their clinical significance .Results The high expression rate of VEGF -D was 60.00%in 60 cases of TSCC , and 25.00%in the control group ( P=0.026 ) .High LVD was observed in 20% in the control group.High peritumoral LVD was detected in 58.33%(P=0.008) of the experimental group .High intratumoral LVD was found in 48.33%(P=0.043) of the 60 cases of TSCC.The expression of VEGF-D and peritumoral LVD was significantly correlated with lymph node metastasis , clinical stage , living conditions and prognosis ( P<0.05 ) .High in-tratumoral LVD was only correlated with the classification of T staging of tumors .The expression of VEGF-D had a posi-tive correlation with peritumoral LVD(r=0.966, P<0.001).Conclusion VEGF-D and lymphangiogenesis are closely correlated with invasion , metastasis and prognosis of TSCC , and they can be important indicators to guide the diagnosis and treatment and predict the prognosis of TSCC .