| HPLC等度洗脱法同时测定银黄薄膜衣片中绿原酸和黄芩苷的含量 |
| |
| 引用本文： | 徐玫,袁琦,赵辉,李帆,呼晓雷,付颖颖,石国华,王晓明,张娜. HPLC等度洗脱法同时测定银黄薄膜衣片中绿原酸和黄芩苷的含量[J]. 中国中药杂志, 2013, 38(5): 691-693 |
| |
| 作者姓名： | 徐玫 袁琦 赵辉 李帆 呼晓雷 付颖颖 石国华 王晓明 张娜 |
| |
| 作者单位： | 河南大学药学院药物分析教研室,河南开封,475001 |
| |
| 基金项目： | 河南大学2010年度大学生创新性实验计划校级项目 |
| |
| 摘 要： | 目的:建立同时测定银黄薄膜衣片中绿原酸和黄芩苷含量的方法.方法:采用HPLC等度洗脱法,C18色谱柱(4.6 mm× 250 mm,5μm),流动相甲醇-水-磷酸(45∶55∶0.2),流速1.0 mL·min-1,柱温30℃,检测波长328 nm,进样量20μL.结果:绿原酸和黄芩苷在2.200 ～44.00 mg·L-1和24.20～242.0 mg·L-1峰面积与进样量呈良好的线性关系,平均回收率分别为99.63％(RSD 1.8％,n=9)和99.72％(RSD 1.9％,n=9).结论:该方法简便、准确,可同时测定银黄薄膜衣片中绿原酸和黄芩苷的含量.
|
| 关 键 词： | 银黄薄膜衣片 绿原酸 黄芩苷 高效液相色谱法 等度洗脱 含量测定 |
| 收稿时间： | 2012-03-15 |
Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution |
| |
| XU Mei,YUAN Qi,ZHAO Hui,LI Fan,HU Xiao-lei,FU Ying-ying,SHI Guo-hu,WANG Xiao-ming and ZHANG Na. Determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets by HPLC isocratic elution[J]. China Journal of Chinese Materia Medica, 2013, 38(5): 691-693 |
| |
| Authors: | XU Mei YUAN Qi ZHAO Hui LI Fan HU Xiao-lei FU Ying-ying SHI Guo-hu WANG Xiao-ming ZHANG Na |
| |
| Affiliation: | Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China;Department of Pharmaceutical Analysis, College of Pharmacy, Henan University, Kaifeng 475001, China |
| |
| Abstract: | |
| |
| Keywords: | Yinhuang film-coated tablets chlorogenic acid baicalin HPLC isocratic elution content determination |
| 本文献已被 万方数据 等数据库收录！ |
|
