转基因厌氧菌对鼠黑色素瘤的靶向性研究

目的探讨转基因婴儿双歧杆菌对小鼠黑色素瘤的靶向性。方法经小鼠尾静脉注射转基因婴儿双歧杆菌，通过放射性体内参入示踪分析、组织厌氧培养及组织切片方法，观察转基因厌氧菌对黑色素瘤组织的靶向性。结果肿瘤组织的放射性强度逐渐增强，而正常组织放射性强度逐渐减弱；组织厌氧培养可见转基因厌氧菌在瘤组织内集聚增殖，组织切片可见瘤组织中有大量的革兰氏染色阳性的条杆状蓝色深染区域，而正常组织则无。结论婴儿双歧杆菌对小鼠黑色素瘤组织具有良好的靶向性，可将携带的基因靶向转运至实体瘤局部。

The research on tumor-targeting of transgenic anaerobic bacterium to melanoma in mice

Objective To evaluate tumor-targeting of transgenic bifidobacterium infants to melanoma in mice. Methods After bolus administration of transgenic bifidobacterium infantis, the values of radioactivity in tumor and organs were examined. Anaerobic culture and histological observation of tumor and normal tissues were taken for examination of tumor-targeting characteristics of transgenic bifidobacterium infantis. Results The radioactivity in melanoma tissue increased progressively, while the radioactivity in normal organs became attenuated with time. The anaerobic culture showed an obvious proliferation of bifidobacterium infantis in tumor tissue. A lame part of area was Gram in the tumor tissue section, whereas the normal tissue was Gram negative. Conclusion Bifidobacterium infantis has good tumor-targeting to melanoma in mice, and recombined gene can be transported technically to solid tumors.