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| 紫外分光光度法测定不同产地半夏中总生物碱的含量 | |
| 引用本文： | 于超,张明,王宇,喻强. 紫外分光光度法测定不同产地半夏中总生物碱的含量[J]. 时珍国医国药, 2002, 13(2): 73-75 |
| 作者姓名： | 于超 张明 王宇 喻强 |
| 作者单位： | 重庆市中药研究院,400065 |
| 摘 要： | 目的；测定不同产地半夏中总生物碱的含量，比较相互差异。方法：对半夏药材采用超声提取，依据酸性染料比色法的原理，在416nm波长下进行比色测定药材中总生物碱。结果：获得良好的线性关系（r=0.9998）和回收率（102.02%），RSD为2.38％。样品在5h内基本稳定（RSD为0.3235%）。结论：测定方法简单快速；不同产地半夏总生物碱含量有一定差异，但相差不大。 |
| 关 键 词： | 半夏 总生物碱 紫外分光光度法 盐酸麻黄碱 化学分析 中药 |
| 文章编号： | 1008-0805(2002)02-0073-02 |
| 修稿时间： | 2001-10-29 |
Determination of the Alkaloids in Pinellia ternata (Thunb.) Breit. from Varied Areas by UV Absorption Spectroscopy |
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| YU Chao,ZHANG Ming,WANG Yu,YU Qiang. Determination of the Alkaloids in Pinellia ternata (Thunb.) Breit. from Varied Areas by UV Absorption Spectroscopy[J]. Lishizhen Medicine and Materia Medica Research, 2002, 13(2): 73-75 | |
| Authors: | YU Chao ZHANG Ming WANG Yu YU Qiang |
| Abstract: | Objective:To determine the alkaloids of Pinellia ternata (Thunb.) Breit. in different area for understanding the difference.Methods:Using the ultrasonic cleaner to extract the herbs, the alkaloid was tested at 416 nm with the spectroscopy by acid dye.Results:The calibration curves of alkaloid were linear (r=0.999 8). The recovery was perfect (102.02%). The samples were stabilization in 5 hours.Conclusion:This method is simple and quick. There is difference in the amount of alkaloids from diferent areas. |
| Keywords: | Pinellia ternata (Thunb.) Breit. Alkaloid UV absorption spectroscopy Ephedrine hydrochloride |
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