| HPLC法测定小儿智力糖浆中细叶远志皂苷的含量 |
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| 引用本文： | 李新娥,石尧辉. HPLC法测定小儿智力糖浆中细叶远志皂苷的含量[J]. 中国医药导报, 2011, 8(19): 60-61 |
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| 作者姓名： | 李新娥 石尧辉 |
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| 作者单位： | 1. 湖南省湘潭市第一人民医院药剂科,湖南湘潭,411101
2. 湖南新汇制药有限公司,湖南长沙,410200 |
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| 摘 要： | 目的：建立小儿智力糖浆中细叶远志皂苷的含量测定方法。方法：采用HPLC法测定。色谱柱：E2111646Z C18柱（4.6 mm×250 mm,5μm）,流动相：甲醇-0.05%磷酸溶液（70∶30）,流速：1.0 ml/min,检测波长：210 nm,理论板数按细叶远志皂苷峰计算不应低于3 000。结果：细叶远志皂苷在30.4～608μg/ml范围内呈良好的线性关系;平均加样回收率为99.20%,RSD=0.95%（n=5）。结论：该方法简便,结果准确,重现性好,可作为小儿智力糖浆质量控制方法。
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| 关 键 词： | 小儿智力糖浆 远志 细叶远志皂苷 HPLC 含量测定 |
Determination of Tenuifolin in the children intelligence syrup by HPLC |
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| LI Xin'e,SHI Yaohui. Determination of Tenuifolin in the children intelligence syrup by HPLC[J]. China Medical Herald, 2011, 8(19): 60-61 |
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| Authors: | LI Xin'e SHI Yaohui |
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| Affiliation: | 1.Pharmacy Department Office,the First People’s Hospital,Xiangtan 411100,China;2.Hu’nan Xinhui Pharmaceutical Co.Ltd.,Changsha 410200,China |
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| Abstract: | Objective：To establish a method for determination of the tenuifolin in the children intelligence syrup by HPLC.Methods：E2111646Z C18 column（4.6 mm ×250 mm,5 μm） was used,the mobile phase consisted of methanol-0.05% phosphoric acid solution（70∶30,V/V）,flow rate was 1.0 ml/min,and the detective wavelength was 210 nm,theoretical tower number calculated by tenuifloin peak should not under the level of 3 000.Results：There were good straightness performance when the concentration of tenuifolin between 30.4 to 608 μg/ml.The average recovery of tenuifolin was 99.20% and the RSD was 0.95%（n=5）.Conclusion：This method is simple and convenient.The accurate result and reappearing feature will be regarded as the quality control method of children intelligence syrup. |
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| Keywords: | HPLC |
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