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Essential role of mitochondrial Ca2+-activated and ATP-sensitive K+ channels in sildenafil-induced late cardioprotection
Authors:Wang Xiaoyin  Fisher Patrick W  Xi Lei  Kukreja Rakesh C
Affiliation:Division of Cardiology, Department of Internal Medicine, Box 980204, Virginia Commonwealth University, 1101 East Mashall Street, Room 7-046, Richmond, VA 23298-0204, USA
Abstract:
Sildenafil (Viagra), a phosphodiesterase type-5 inhibitor used in treatment of male erectile dysfunction and pulmonary hypertension can induce cardioprotection through opening of mitochondrial ATP-sensitive K+ channels (mitoKATP). Recent studies suggest that activation of mitochondrial Ca2+-activated K+ channels (mitoKCa) also has anti-ischemic effects. However, the relative role of mitoKCa and mitoKATP in sildenafil-induced cardioprotection remains unknown. In the present study, adult male ICR mice were pretreated with sildenafil (0.71 mg/kg, i.p.) 24 h prior to 20 min of global ischemia followed by 30 min of reperfusion in Langendorff mode. Paxilline (blocker of KCa) or 5-hydroxydecanoic acid (5-HD; blocker of mitoKATP) was administered either 30 min before sildenafil or 10 min prior to ischemia. Treatment with sildenafil reduced infarct size, which was abolished by either paxilline or 5-HD. Furthermore, in vivo gene knockdown of β1 subunit of KCa (KCa-β1) using small interfering RNA (siRNA) administered 48 h before sildenafil injection blocked the infarct limiting effect of sildenafil. The protective effect of sildenafil was preserved in mice treated with non-target siRNA. Western blots demonstrated selective protein expression of KCa-β1 in cardiac mitochondria and the gene knockdown effect of siRNA on KCa-β1. The level of KCa-β1 protein was not upregulated following treatment with sildenafil. We conclude that both mitoKCa and mitoKATP play a critical role in triggering and mediating sildenafil-induced delayed cardioprotection. The results suggest that activation of mitoKCa and mitoKATP are crucial for maintaining mitochondrial homeostasis and reducing cell death in sildenafil-induced preconditioning against ischemia-reperfusion injury.
Keywords:Phosphodiesterase inhibitor   Potassium channel   Ischemia-reperfusion   Cardioprotection   Small interfering RNA
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