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| 医院感染铜绿假单胞菌产超广谱β-内酰胺酶基因型研究 | |
| 引用本文： | 徐卫,金嵘,石娜,方晔,韩珍,周铁丽. 医院感染铜绿假单胞菌产超广谱β-内酰胺酶基因型研究[J]. 上海医学检验杂志, 2009, 0(1) |
| 作者姓名： | 徐卫 金嵘 石娜 方晔 韩珍 周铁丽 |
| 作者单位： | 温州医学院附属第一医院;温州医学院; |
| 基金项目： | 温州医学院重大科研课题B类项目基金资助(EXYZD-B2005-08) |
| 摘 要： | 目的研究医院感染铜绿假单胞菌产超广谱β-内酰胺酶(ESBLs)的基因型分布。方法用聚合酶链反应(PCR)对68株医院感染铜绿假单胞菌的SHV、TEM、CTX-M、OXA-10、VEB和PER等基因进行扩增和序列测定。结果68株医院感染铜绿假单胞菌中,检出26株产OXA-10群ESBLs,检出率为38.23%(26/68);其中1株既产OXA-10群ESBLs又产TEM型广谱β-内酰胺酶;其余基因型未检出。经序列测定为OXA-10-like基因和TEM-1基因。结论医院感染铜绿假单胞菌所产的ESBLs主要为OXA-10群,且为多重耐药菌株。 |
| 关 键 词： | 铜绿假单胞菌 超广谱β-内酰胺酶 基因型 医院感染 |
Study on the genotype of extended spectrum beta-lactamases among Pseudomonas aeruginosa in nosocomial infection |
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| XU Wei,JIN Rong,SHI Na,FANG Ye,HAN Zhen,ZHOU Tieli.. Study on the genotype of extended spectrum beta-lactamases among Pseudomonas aeruginosa in nosocomial infection[J]. Shanghai Journal of Medical Laboratory Sciences, 2009, 0(1) | |
| Authors: | XU Wei JIN Rong SHI Na FANG Ye HAN Zhen ZHOU Tieli. |
| Affiliation: | 1.The First Affiliated Hospital;Wenzhou Medical College;Zhejiang Wenzhou 325000;China;2.Wenzhou Medical College;China |
| Abstract: | Objective To study the genotype of extended spectrum beta-lactamases(ESBLs) among Pseudomonas aeruginosa in nosocomial infection.Methods SHV,TEM,CTX-M,OXA-10,VEB and PER gene of 68 nosocomial isolates of Pseudomonas aeruginosa were detected by polymerase chain reaction(PCR).Some of the products were sequenced.Results Among nosocomially isolated 68 Pseudomonas aeruginosa,26 strains had the OXA-10 groups ESBLs gene with a positive rate of 38.23%(26/68),and one strain had both OXA-10 groups ESBLs and TEM broad... |
| Keywords: | Pseudomonas aeruginosa Extended spectrum beta-lactamase Genotype Nosocomial infection |
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