| A COMPARATIVE STUDY OF DNA FLOW CYTOMETRY AND MICROSPECTROPHOTOMETRY IN GASTRIC CARCINOMAS |
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| 作者姓名： | 庄亚华 王瑞年 刘炳荣 魏仁志 林言箴 |
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| 作者单位： | Department of Pathology，College of Basic Medical Sciences，SSMU，Shanghai，Department of Pathology，College of Basic Medical Sciences，SSMU，Shanghai，Department of Laser Medicine，College of Basic Medical Sciences，SSMU，Shanghai，Department of Surgery，Ruijin Hospital，SSMU，Shanghai，Department of Surgery，Ruijin Hospital，SSMU，Shanghai |
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| 摘 要： | Flow cytometric (FCM) and microspectrophotometric (MSP) measurements of cell nuclear DNA content were made in 53 fresh gastric carcinoma specimens and in 30 gastric mucosal specimens with chronic gastritis. DNA aneuploidy was found in 32/53 (60%) of gastric carcinomas, and appeared more frequently in wellor moderately differentiated tubular adenocarcinomas (90%) than in undifferentiated and mucousa cell carcinomas (23.6%) (P<0.001). No aneuploidy was found in chronic gastritis samples, but their proliferative cell fractions were higher than in normal control gastric mucosa samples (P<0.01). A comparison was made between FCM and MSP analyses of DNA content in 29 cases of gastric carcinoma, a high correlation rate (r=0.90) was found. The advantages and limitations of both methods are discussed, they may be used in combination for more precise cytochemical analysis.
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A COMPARATIVE STUDY OF DNA FLOW CYTOMETRY AND MICROSPECTROPHOTOMETRY IN GASTRIC CARCINOMAS |
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| Zhuang Yahua Wang Ruinian.A COMPARATIVE STUDY OF DNA FLOW CYTOMETRY AND MICROSPECTROPHOTOMETRY IN GASTRIC CARCINOMAS[J].Journal of Shanghai Second Medical University(Foreign Language Edition),1990(1). |
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| Authors: | Zhuang Yahua Wang Ruinian |
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| Institution: | Zhuang Yahua Wang Ruinian Department of Pathology,College of Basic Medical Sciences,SSMU,Shanghai Liu Bingrong Department of Laser Medicine,College of Basic Medical Sciences,SSMU,Shanghai Wei Renzhi Lin Yenzheng Department of Surgery,Ruijin Hospital,SSMU,Shanghai |
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| Abstract: | Flow cytometric (FCM) and microspectrophotometric (MSP) measurements of cell nuclear DNA content were made in 53 fresh gastric carcinoma specimens and in 30 gastric mucosal specimens with chronic gastritis. DNA aneuploidy was found in 32/53 (60%) of gastric carcinomas, and appeared more frequently in wellor moderately differentiated tubular adenocarcinomas (90%) than in undifferentiated and mucousa cell carcinomas (23.6%) (P<0.001). No aneuploidy was found in chronic gastritis samples, but their proliferative cell fractions were higher than in normal control gastric mucosa samples (P<0.01). A comparison was made between FCM and MSP analyses of DNA content in 29 cases of gastric carcinoma, a high correlation rate (r=0.90) was found. The advantages and limitations of both methods are discussed, they may be used in combination for more precise cytochemical analysis. |
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| Keywords: | flow cytometry microspectrophotometry DNA ploidy gastric carcinoma |
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