反义寡核苷酸防治移植物动脉硬化的作用

目的　探讨防治移植物动脉硬化的途径。方法　通过大鼠胸腹主动脉移植简化模型 ,用各种增殖细胞核抗原 (PCNA)寡核苷酸在脂质体介导下转染大鼠胸主动脉后 ,移植到大鼠腹主动脉 ,于术后 15、3 0和 60d取移植动脉作病理学检查及PCNA逆转录PCR检测。结果　病理学结果示 ,在 3个时相点 ,反义寡核苷酸组移植动脉再狭窄率 (8.3 %、12 .4%、19.5 % )均显著低于对照组 (P <0 .0 5 )。逆转录PCR结果显示 ,在 3个时相点 ,反义寡核苷酸组PCNAmRNA的表达(5 9.2 4、80 .16、18.5 8)均明显低于对照组 (P >0 .0 5 )。结论　PCNA反义寡核苷酸可明显抑制移植动脉PCNA蛋白的表达 ,抑制动脉内膜增生 ,防治移植物动脉硬化。

Prevention of graft arteriosclerosis by proliferating cell nuclear antigen antisense oligonucleotide

Objective To explore a pathway to prevent the graft arteriosclerosis.Methods The thoracic aorta of rats transfected with different PCNA oligonucleotides mediated by liposomes was transplanted to abdominal aorta in the single model of thoracic aorta to abdominal aorta.The pathological examination and RT-PCR of PCNA were carried out at 15,30 and 60 day after the operation.Results The pathological results showed that the restenosis rates (8.3 %,12.4 %,19.5 % at 15,30 and 60 days respectively) in the group of antisense oligonucleotide were all lower than those in the control groups (P<0.05).The RT-PCR results showed that the expression of PCNA mRNA (59.24,80.16,18.58 at 15,30 and 60 days respectively) in the group of antisense oligonucleotide were all lower than those in the control groups (P<0.05).Conclusion The antisense oligonucleotide of PCNA can obviously suppress the expression of PCNA protein in the transplanted aorta and the hyperplasia of intima,and prevent the graft arteriosclerosis.