复方丹参注射液对脊髓损伤大鼠细胞凋亡的影响

目的:通过观察复方丹参注射液对急性脊髓损伤大鼠治疗前后神经功能评分及脊髓细胞凋亡率等的影响,探讨复方丹参注射液对大鼠脊髓损伤细胞凋亡的保护作用及作用机制。方法:采用Allen法将64只SD大鼠制成脊髓中度损伤模型,分别给予复方丹参注射液、甲基强的松龙、亚胺环己酮及生理盐水治疗,观察比较治疗前后动物不同时间的神经功能评分、斜板试验及脊髓细胞凋亡率的变化。结果:从伤后2周和3周起,治疗组的神经功能评分和斜板最大角度分别与对照组比较差异均有显著性(P<0.01),而治疗组间差异无显著性(P>0.05)。从伤后1周起,治疗组的细胞凋亡率与对照组比较差异均有显著性(P<0.05)。结论:复方丹参注射液对大鼠脊髓损伤细胞凋亡具有保护作用。

Effect of compound Tanshin injection on cell apoptosis in rats with acute spinal cord injury

AIM:To observe the effect of compound tanshin injection on cell apoptosis and nerve function before and after treatment in rats with acute spinal cord injury and to investigate its protective effect and action mechanism.METHODS:Sixty four rats were enrolled to establish moderate spinal cord injury models with Allen method, and then were equally divided into 4 groups at random, in which they received compound tanshin injection, methylprednisolone, cycloheximide or physiological saline treatment respectively. Tarlov scale and inclined plane test were conducted in the four groups, and apoptosis rate were examined with flow cytometer at 1,2,3,4 and 6 weeks after injury.RESULTS:From the second and third week, significant differences(P< 0.01)were shown between the treated groups and controlled group in Tarlov scale and inclined plane test respectively.From the first week,significant differences(P< 0.05)were shown between the treated groups and controlled group in apoptosis.