F-box蛋白4和端粒重复序列结合因子1相互作用核蛋白2mRNA表达与食管鳞癌发生发展关系研究

目的 检测食管鳞癌组织中F-box蛋白4(FBX4)和端粒重复序列结合因子1相互作用核蛋白2(TIN2)mRNA表达,并分析其与食管鳞癌相关临床指标的关系.方法 收集110例明确诊断的食管鳞癌中心组织及其30例癌旁组织,采用SBYR Green实时荧光定量PCR方法检测FBX4和TIN2基因在食管鳞癌中心组织和癌旁组织mRNA水平的表达,并分析FBX4和TIN2 mRNA表达临床相关指标的关系.结果 FBX4 mRNA在食管鳞癌组织中的相对表达量为0.24±0.43(n=110),而在肿瘤旁组织中为0.40±0.27(n=30),两者差异具有统计学意义(P=0.008);食管鳞癌组织中TIN2 mRNA的相对表达量为1.59±1.19(n=101);肿瘤旁为2.54±1.89(n=30),两者差异具有统计学意义(P=0.000).TIN2 mRNA表达在临床各参数不同分组内均未发现有统计学差异(P>0.05),而FBX4 mRNA表达虽在不同性别、年龄、钡餐分型、临床分期和肿瘤长度中均没有统计学差异(P>0.05),但在不同病理分型中存在统计学差异,即相对于高分化鳞癌,低分化和中分化的鳞癌组织中的FBX4 mRNA表达增加,差异具有统计学意义(P<0.05).FBX4和TIN2在肿瘤组织中的表达呈正相关(r=0.671,P=0.000).结论 FBX4和TIN2 mRNA在食管鳞癌组织中均表现表达增高,且存在直线相关关系,提示FBX4和TIN2可能在食管癌的发生发展中具有重要的临床意义;肿瘤分化程度与FBX4表达相关,而与TIN2不相关,表明肿瘤分化还与FBX4介导的泛素化相关.

Studies on the relationship of the expressions of TIN2 and FBX4 mRNA with the carcinogenesis of esophageal cancer

Objective To detect the expressions of TIN2 and FBX4 mRNA in esophageal squamous cell carcinoma, and analyze the relationship of esophageal squamous cell carcinoma associated with clinical indicators . Methods 110 cases of esophageal squamous cell carcinoma and 30 cases of its adjacent tissues were collected , using SBYR Green fluorescent quantitative PCR assay to detect the mRNA expression of FBX 4 and TIN2 genes in esophageal squamous cell cancer, and analysis the relationship between the mRNA expression of FBX 4 and TIN2 and clinically relevant indicators. Results The relative expression of FBX4 mRNA in esophageal squamous cell carcinoma was O. 24± 0. 43 （n = l IO）, while in its adjacent tissues was 0. 40 ± O. 27 （n = 30）, the difference had statistical significance（P =0. 008 ）;the relative expression of TIN2 mRNA in esophageal squamous cell carcinoma was 1.59 ± 1.19 （ n = 101 ） ; in its adjacent tissues was 2. 54 ± 1.89 （ n = 30 ）, the difference had statistical significance （ P = 0. 000 ）. TIN2 mRNA expression and various clinical parameters had not found significant difference （P 〉 0. 05 ）, while FBX4 mRNA expression, although with gender, age, }~arium meal type, clinical stage and tumor length were not significantly different （ P 〉 0. 05 ）. However, with the pathological type, there was a significant difference, which was compared with well-differentiated squamous cell carcinoma, FBX4 mRNA expression was significantly increased in poorly differentiated and middle differentiated squamous cell carcinoma tissues , with statistical siguificanee （ P 〈 0. 05 ）. Conclusions FBX4 and TIN2 mI{NA in esophageal squamous cell carcinoma showed increased expression, and there is a linear correlation between them , suggesting FBXa and TIN2 in esophageal cancer development may have important clinical significance ; degree of tumor differentiation eonelated with FBX4 expression but rmt with TIN2 suggests tumor differentiation may be related with FBX 4 that mediated ubiquitination.