VEGF和微血管密度与大肠癌肝转移的关系

目的探讨血管内皮细胞生长因子（VEGF）和微血管密度（MVD）与大肠癌肝转移的关系。方法收集88例大肠癌患者的临床病理资料，检测VEGF血浆浓度、肿瘤组织含量，进行免疫组织化学染色，并计数微血管密度。结果血浆VEGF浓度与肝转移及TNM分期显著相关，肿瘤组织含量与肿瘤浸润深度、淋巴结转移、肝转移及TNM分期显著相关，VEGF染色阳性率与肝转移显著相关。VEGF和MVD正相关。术后肝转移患者的血浆VEGF浓度、肿瘤组织含量和染色阳性率以及MVD均显著高于无肝转移病例。结论VEGF和MVD与大肠癌的进展相关，是术后肝转移的预测指标。

Relationships of VEGF and microvessel density with liver metastasis in human colorectal cancer

Objective To investigate the relationship of vascular endothelial growth factor （VEGF） and microvessel density （MVD） with liver metastasis in colorectal cancer. Methods In 88 cases of colorectal cancer, preoperative plasma levels and tumor tissue content of VEGF were assessed by using ELISA - kits. Tissue samples were also immunohistochemically examined for the VEGF and FVⅢ/RA expression and counting for MVD was performed by using Weidner method. Results There were significant associations between plasma VEGF levels and liver metastasis and TNM stage. Tumor tissue VEGF content was correlated to tumor invasion,lymph nodal involvement,liver metastasis and TNM stage. A significant correlation was found between the expression of VEGF and liver metastasis. The positive correlation between VEGF and MVD was also detected. Preoperative plasma VEGF levels, tumor VEGF content, VEGF positive rate and MVD were higher in patients with liver metastasis than in those without liver metastasis. Conclusion In human colorectal carcinoma, VEGF and MVD are significantly associated with tumor progression and may be useful predictors for liver metastasis.