广藿香中的黄酮类化合物

目的 对广藿香全草进行研究以筛选天然抗真菌新药。方法 用色谱技术进行分离，通过IR，UV，MS，NMR（^1H,^13C,DEPT）分析以及与标准品对照的方法鉴定化合物的结构。利用培养基药物浓度稀释法进行体外抗菌实验。结果 利用抗菌活性追踪，从广藿香中分离得到8个黄酮类化合物，鉴定为：5-羟基-3‘,7,4‘-三甲氧基二氢黄酮（I）；5-羟基-7,4‘-二甲氧基二氢黄酮（Ⅱ)；3,5-二羟基-7,4‘-二甲氧基黄酮（Ⅲ)；5-羟基-3‘,7,4‘-三甲氧基黄酮（IV）；5-羟基-3‘,7,3‘4‘-四甲氧基黄酮（V）；5,4‘-二羟基-3,7,3‘-三甲氧基黄酮（VI) ;5,4‘-二羟基-7-甲氧基黄酮（Ⅶ)和3,5,7,3‘,4‘-五羟基黄酮（Ⅶ)。并对化合物I,Ⅲ,Ⅴ,Ⅵ进行了体外抗真菌活性研究。结论 除V和VI外，均为首次从该植物中分离得到。受测的4个黄酮类化合物具有抗真菌活性。

Flavonoids isolated from Pogostemon cablin

Objecl To isolate and identify the chemical constituents of Pogostemon cablin (Blanco)Benth. For the purpose to screen new natural antifungal drugs. Methods The compounds were isolated by chromatography. Their chemical structures were elucidated with UV, IR, MS, NMR (1H, 3C, DEPT) spectroscopy. The antifungal activities of the four isolated compounds were examined in vitro by media dilution method. Results With antifungal guided fractionation, eight flavonoids were isolated from the alcoholic extract of the whole plant. Their structures were elucidated as 5-hydroxy-7, 3′, 4′-trimethoxyflavanone ( Ⅰ ), 5-hydro,xy-7, 4′-dimethoxyflavanone ( Ⅱ ), 3, 5,-dihydroxy-7, 4′-dimethoxyflavone ( Ⅲ ),5-hydroxy-3, 7, 4′-trimethoxyflavone (Ⅳ), 5-hydroxy-3, 7, 3′, 4′-tetramethoxyflavone (Ⅴ), 5, 4′-di-hydroxy-3, 7, 3′-trimethoxyflavone (Ⅵ), 5, 4′-dihydroxy-7-ethoxyflavone (Ⅶ), and 3, 5, 7, 3′, 4′-pentahydroxyflavone (Ⅷ) on the basis of spectroscopic data. The antifungal activities of compounds Ⅰ ,Ⅲ , Ⅴ , Ⅵl were exanined in vitro. Conclusion All the flavonoids, except Ⅴ and Ⅵ were isolated from this plant for the first time. The four examined compounds exhibited certain antifungal activities against six strains of fungi in vitro.