糖尿病大鼠肾脏细胞凋亡与bcl-2基因表达

目的　探讨糖尿病大鼠肾脏细胞凋亡的发生及其细胞凋亡与糖尿病肾病之间的关系。方法　大鼠腹腔注射链脲佐菌素 (STZ)诱发糖尿病模型 ,分别在动物模型建立后第 4、8、12、2 0周 ,利用原位末端标记法 (TUNEL)观察肾脏细胞凋亡情况 ,以免疫组化方法检测凋亡相关基因bcl 2的表达 ,并检测尿素氮、血肌酐等反映肾功能的有关指标。结果　糖尿病组肾小管凋亡细胞数较同期对照组明显增多 ,肾小球在 8周时开始出现凋亡细胞 ,后逐渐增多 ,对照组肾小球未见凋亡细胞。与对照组相比 ,bcl 2基因表达减弱 ,肾脏细胞凋亡数与bcl 2表达具有相关性 ( P <0 0 5 )。结论　肾脏凋亡细胞的不断增加可能是糖尿病肾病发生、发展的原因之一 ,bcl 2参与肾脏细胞凋亡的调控

Apoptosis and expression of bcl-2 in the kidney of diabetic rats

Objective: To investigate the relationship between apoptosis and the expression of bcl、2 in the kidney of diabetic rats. Methods: Diabete was induced by intraperitoneal injection of STZ(65 mg/kg). After 4, 8, 12 and 20 weeks respectively, apoptosis was identified by terminal、deoxynucleotidyl transferase mediated d、UTP nick end labeling (TUNEL). Immunohistochemistry was used to detect apoptosis associated gene expression of bcl、2. Profiles about renal functions such as BUN and Scr were detected. Results: Apoptic cell obviously increased in tubules during 20 weeks and detected in glomeruli from 8 weeks and progressively increased with deterioration of renal functions. Compared with those in the kidneys of control group, the level of expression of bcl、2 decreased, Furthermore, the number of apoptic cells and expression of bcl、2 were significantly correlated. Conclusion: Apoptos in the diabetic kidney may play a role in the progression of diabetic nephropathy. The change in bcl、2 activities may be responsible for apoptosis in the diabetic kidney.