T淋巴细胞亚群及过敏原与婴幼儿肺炎支原体感染伴喘息的关系

目的 分析肺炎支原体 （MP）感染伴喘息婴幼儿的T淋巴细胞亚群表达及过敏原筛查情况。方法 流式细胞仪检测354例MP感染伴喘息婴幼儿 （MP喘息组）、336例MP感染不伴喘息婴幼儿 （MP非喘息组）、277例反复喘息患儿 （反复喘息组）的外周血T淋巴细胞亚群表达，同时进行过敏原检测。结果 MP喘息组和反复喘息组的CD3+及CD3+CD8+淋巴细胞百分比均低于MP非喘息组 （P < 0.05）；MP喘息组和MP非喘息组的CD3+CD4+淋巴细胞百分比均高于反复喘息组 （P < 0.05）；MP喘息组和反复喘息组的CD3-CD19+及CD19+CD23+淋巴细胞百分比均明显高于MP非喘息组 （P < 0.05），以反复喘息组最高 （P < 0.05）。食入性过敏原检测总阳性率 （30.3%）高于吸入性过敏原 （14.7%），P < 0.05；反复喘息组、MP喘息组的食入性和吸入性过敏原阳性率均高于MP非喘息组，以反复喘息组最高 （P < 0.05）。结论 T淋巴细胞亚群紊乱、过敏体质在MP感染伴喘息的婴幼儿发病起着重要作用。

Association of T lymphocyte subsets and allergens with Mycoplasma pneumoniae infection complicated by wheezing in infants and young children

Objective To investigate the percentage of T lymphocyte subsets and allergen screening results in infants and young children with Mycoplasma pneumoniae (MP) infection complicated by wheezing. Methods Flow cytometry was used to measure the percentage of peripheral blood T cell subsets in 354 infants and young children with MP infection complicated by wheezing (MP wheezing group), 336 infants and young children with MP infection but without wheezing (MP non-wheezing group), and 277 children with recurrent wheezing (recurrent wheezing group). Allergen screening was also performed for these children. Results Both the MP wheezing group and recurrent wheezing group had signiifcantly lower percentages of CD3+and CD3+CD8+lymphocytes than the MP non-wheezing group (P<0.05). The MP groups with or without wheezing had a significantly higher percentage of CD3+CD4+lymphocytes than the recurrent wheezing group (P<0.05). Both the MP wheezing group and recurrent wheezing group had signiifcantly higher percentages of CD3-CD19+and CD19+CD23+lymphocytes than the MP non-wheezing group (P<0.05), and the recurrent wheezing group had the highest percentages (P<0.05). The overall positive rate of food allergens was signiifcantly higher than that of inhaled allergens (30.3%vs 14.7%;P<0.05). The positive rates of food and inhaled allergens in the recurrent wheezing group and MP wheezing group were signiifcantly higher than in the MP non-wheezing group (P<0.05), and the recurrent wheezing group had the highest rates. Conclusions Imbalance of T lymphocyte subsets and allergic constitution play important roles in the pathogenesis of MP infection complicated by wheezing in infants and young children.