全蝎醇提物对慢性癫痫模型大鼠海马GFAP mRNA表达的影响

目的：研究全蝎醇提物（EES）对慢性癫痫模型大鼠海马胶质纤维酸性蛋白（GFAP）mRNA表达的影响。方法：大鼠首日腹腔注射氯化锂（Licl）127mg·kg^-1（3mmol·kg^-1）,18～24h后腹腔注射硫酸阿托品1mg·kg^-1,30min后腹腔注射毛果芸香碱（Pilo）30mg·kg^-1,以复制慢性癫痫模型。实验分为正常对照（等容生理盐水）、模型（等容生理盐水）、丙戊酸（120mg·kg^-1）和EES高、中、低剂量（1160、580、290mg·kg^-1）组。观察各组大鼠慢性癫痫发作级别情况,用RT-PCR方法测定大鼠癫痫持续状态后6h和1、3、7、14、30d各时点海马GFAP mRNA的表达情况。结果：与模型组比较,EES高、中剂量组大鼠慢性癫痫发作级别有显著性差异（P〈0.05）。在复制模型后3d大鼠海马GFAP mRNA表达明显上调,7d达峰值,然后逐渐下调,但仍显著高于正常对照组。给药7d时,与模型组比较,EES高、中剂量组GFAP mRNA表达显著下调（P〈0.05）。结论：高、中剂量EES能降低慢性癫痫模型大鼠海马GFAP mRNA的表达,可能是EES发挥抗癫痫作用的机制之一。

Effects of Ethanol Extract of Scorpion on the mRNA Expression of Hippocampus GFAP in Rats with Chronic Epilepsia

OBJECTIVE： To study the effects of ethanol extract of Scorpion （EES） on mRNA expression of hippocampus GFAP in rats with chronic epilepsia. METHODS： Rats were injected with lithium chloride （Licl） 127 mg·kg^-1（3 mmol. kg^-1） and atropine sulfate 1 mg·kg^-1 for 18-24 h intraperitoneally on the first day. 30 min later, they were given pilocarpine （Pilo） 30 mg·kg^-1 intraperitoneally to induce chronic epilepsia model. Model rats were randomly divided into normal control group（isovolumic saline）, model group （isovolumic saline）, valproic acid group （ 120 mg·kg^-1）, EES low-dose （290mg·kg^-1）, medium-dose （580 mg·kg^-1）and high-dose （1 160 mg·kg^-1） groups. The degree of chronic epilepsia attack in rats was observed, and the mRNA expression of hippocampus GFAP was observed by RT-PCR at 6 hour and 1 day, 3 day, 7 day, 14 day and 30 day after status epilepticus. RESULTS：Compared with model group, epileptic seizures grading was significantly changed in EES high-dose group and EES medium dose group.3 days after modeling, mRNA expression of hippocampus GFAP in rats was up-regulated significantly, reached to the peak 7 days later, and then declined gradually, which was still higher than normal control group. Compared with model group, GFAP mRNA contents had declined significantly in EES high-dose and medium-dose groups after 7 days of treatment（P〈 0.05）. CONCLUSION： High-dose and medium-dose of EES can reduce the mRNA expression of hippocampus GFAP in rats with chronic epilepsia, which is an important anti-epileptic mechanism.