| 中药材龟板和鳖甲中DNA的提取与扩增 |
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| 引用本文： | 王亚明,周开亚,吴平,徐珞珊.中药材龟板和鳖甲中DNA的提取与扩增[J].药学学报,1996,31(6):472-476. |
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| 作者姓名： | 王亚明 周开亚 吴平 徐珞珊 |
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| 作者单位： | 南京师范大学生物系，中国药科大学生药学研究室 |
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| 摘 要： | 中药材龟板和鳖甲中ＤＮＡ的提取与扩增王亚明，周开亚，吴平，徐珞珊（南京师范大学生物系，南京２１００９７；中国药科大学生药学研究室，南京２１０００９）龟鳖类药材在我国使用历史悠久，具补阴益气的功效。《中国药典》（１９９０年版）规定龟甲（龟板）的原动物为...
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| 关 键 词： | 龟板 鳖甲 DNA提取 PCR扩增 |
| 收稿时间： | 1995-06-15 |
ISOLATION AND AMPLEFICATION OF DNA FROM TORTOISE AND TURTLE SHELLS |
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| YM Wang,KY Zhou,P Wu and LS Xu.ISOLATION AND AMPLEFICATION OF DNA FROM TORTOISE AND TURTLE SHELLS[J].Acta Pharmaceutica Sinica,1996,31(6):472-476. |
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| Authors: | YM Wang KY Zhou P Wu and LS Xu |
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| Abstract: | DNA was extracted from tortoise and turtle shells, two Chiense crude drugs whichhave been preserved for at least nine years.The cytochrome b sequence was amplified from theextracts using the polymerase chain reaction,Treatments with detergent and ultraviolet light wereemployed to destroy possible extraneous contamination,A control extract was set in parallel with theextracts of the specimens in order to detect contamination in solutions and reagents.The results showthat significant amounts of genetic information ean survive in the shells and may have importantimpllcations for their identification. |
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| Keywords: | Tortoise shell Turtle shell DNA isolation PCR amplification |
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